https://repository.kopri.re.kr/handle/201206/5379 Mon, 06 Apr 2026 03:33:57 GMT 2026-04-06T03:33:57Z https://repository.kopri.re.kr/handle/201206/13643 Title: Application of Column Chromatography for Accurate Determination of the Carbon Isotopic Compositions of n-alkanes in Diverse Environmental Samples Authors: Lee, Dong-Hun; Kim, Jung-Hyun; Shin, Kyung-Hoon Abstract: The carbon isotopic compositions (δ13C) of n-alkanes in various environmental samples have been previously proposed as suitable fngerprints for assessing the origin of organic matter (OM) in diverse environmental systems. However, with respect to using gas chromatography？combustion？isotope ratio mass spectrometry for the carbon isotopic analysis of n-alkanes, analytical uncertainty may often be caused by the co-elution of interfering unsaturated compounds (e.g., aromatic and branched compounds). Hence, we propose a simple but reliable method for purifcation that uses column chromatography. The performance of two diferent solid stationary phases (i.e., aluminum oxide and Ag+-impregnated silica) was compared in terms of their capacity to eliminate unsaturated compounds from total hydrocarbons and thus increase the precision of δ13C measurements. Compared to the use of an activated aluminum oxide column, elution from an Ag+-impregnated silica column allows more efective isolation of individual n-alkanes, which results in more precise δ13C measurements for diverse environmental samples. Thus, Ag+-impregnated silica column separation can be efective as a routine experimental technique for increasing the accuracy of the δ13C values for n-alkanes in OM, which includes a large proportion of unsaturated compounds. Mon, 01 Mar 2021 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/13643 2021-03-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/10970 Title: Bacterial endosymbiont of Oligobrachia sp. (Frenulata) from an active mud volcano in the Canadian Beaufort Sea Authors: Lee, Yung Mi; Noh, Hyun-Ju; Lee, Dong-Hun; Kim, Junghyun; Jin, Young Keun; Paull, Charles Abstract: Siboglinid tubeworms of the genus Oligobrachia that thrive in obligatory association with endosymbionts have been predominantly observed in Arctic and high-latitude Atlantic cold seeps. Metabolic features of endosymbionts provide fundamental understanding for the survival strategy of tubeworms in cold seeps. However, no information on the bacterial endosymbionts of tubeworms from the Canadian Beaufort Sea was available until now. In this study, we investigated the phylogeny and metabolic potential of a bacterial endosymbiont of siboglinid tubeworms from an active mud volcano in the Canadian Beaufort Sea using Illumina MiSeq sequencing of 16S rRNA gene amplicons. The siboglinid tubeworm shared 99.9% 18S rRNA gene sequence similarity with Oligobrachia haakonmosbiensis and 99.7%？99.8% mitochondrial cytochrome C oxidase subunit I gene (COI) similarity with members of Oligobrachia sp. CPL-clade and was designated 'Oligobrachia sp. BS1'. The endosymbiont of Oligobrachia sp. BS1, which belongs to the Gammaproteobacteria, was most closely related to endosymbionts of Oligobrachia sp. CPL-clade, revealing the close relationships between the endosymbionts and their hosts. The bacterial endosymbiont of Oligobrachia sp. BS1 contained the key gene required for sulfur oxidation, aprA gene encoding the α-subunit of adenosine 1,5-phosphosulfate reductase, suggesting that this endosymbiont is capable of using sulfide as an energy source. The bacterial endosymbiont of an Oligobrachia species from an active mud volcano in the Canadian Beaufort Sea presented here expands our knowledge of the identities and thiotrophic metabolism of endosymbionts that are associated with hosts that dominate a wide range of methane seep habitats in the Arctic. Sun, 01 Dec 2019 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/10970 2019-12-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/8557 Title: ARA08C Cruise Report: 2017 Korea-Canada-USA Beaufort Sea Research Program Authors: Jin, Young Keun; Shipborad scientific party Abstract: Barrow, USA-Beaufort Sea-Nome, USA August 27- September 16, 2017 Sun, 01 Apr 2018 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/8557 2018-04-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/4995 Title: ARA07C Cruise Report : 2016 Korea-Russia-Germany East Siberian Sea Research Program Authors: Jin, Young Keun Abstract: Barrow, USA-East Siberian Sea-Nome, USA August 25 - September 10, 2016 Fri, 17 Nov 2017 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/4995 2017-11-17T00:00:00Z