https://repository.kopri.re.kr/handle/201206/5489 Tue, 21 Apr 2026 17:21:19 GMT 2026-04-21T17:21:19Z https://repository.kopri.re.kr/handle/201206/6289 Title: Evaluation of Manila clam Ruditapes philippinarum as a sentinel species for metal pollution monitoring in estuarine tidal flats of Korea: Effects of size, sex, and spawning on baseline accumulation Authors: Ahn, In-Young; Ji, Jung Youn; Choi, Heeseon J. Sun, 01 Jan 2006 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/6289 2006-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/6637 Title: Bioaccumulation of Heavy Metals in Ruditapes philippinarum Authors: Lee, Yong-Seok; Kho, Weon-Gyu; Kang, Se Won; Choi, Sang-Haeng; Jo, Yonghun; JEONG, KYE HEON; Ahn, In-Young; Park, Hong-Seog; Han, Yeon Soo; Cho, Eun Mi; Byun, In Sun Abstract: The present study was conducted to confirm that a bivalve Ruditapes philippinarum can be used as a biomarker for the monitoring of the heavy metal pollution in the silt of the marine environment. The clams were collected from the silt of Cheonsu-bay, Buheung-ri, and Tan-island of the West Sea, Korea. To observe the normal structures of the target organs (hepatopancreas and gill), they were dissected out for the immunohistochemical study and the electron microscopy with TEM, SEM, and SEM-EDS. The immunohistochemical study showed that the interdiverticular connective tissues of the hepatopancreas, and the outer epithelium of the gill lamellae was strongly reacted to anti-metallothionein (MT), indicating the presence of MT, a metal-binding protein, involved in metal detoxifying process. According to the examinations under the TEM, the epithelial cells of the hepatopancreas of the clams collected from polluted area (Tan-island) showed certain changes such as swollen rER, swollen nuclear envelope and inclusion bodies in the nulcei. In the SEM-EDS analysis, tissue of the hepatopancreas showed relatively higher concentration of S, Zn, and Cd. These elements are supposed to be concerning with the MT-reaction in the hepatopancreas. Considering that the coastal bivalve R. philippinarum showed immediate subcellular responses to heavy metal pollution in the overall experiments conducted, this species might act as one of efficient biomarkers for the heavy metal contamination in the marine environment. Sun, 01 Jan 2006 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/6637 2006-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/6674 Title: Spatial Variation of Heavy Metal Accumulation in Manila clam Ruditapes philippinarum from Some Selected Intertidal Flats of Korea Authors: Ahn, In-Young; Pyo, Sei Hong; Park, Hyun; Choi, Heeseon J.; Ji, Jung Youn Abstract: Spatial variation of heavy metal accumulation was investigated in Manila clam Ruditapes philippinarum collected from several tidal flats. Sediment metal levels varied highly among the sites, which was attributed primarily to differences in Fe and organic carbon contents and in part to grain size. Significant differences in metal concentrations also were found in the clam tissue among the different sampling sites. However, except for a few metals (Mn, Zn, Pb), which showed some elevation, the variations in the clam tissue were not related to the variations in the sediment. This is likely because most metals in filter-feeding herbivores such as R. philippinarum accumulated as a result of feeding on suspended particles such as phytoplankton and organic detritus in the water column, not in bottom sediment. In addition, tissue weight for a specific shell size varied significantly among the sites, and increased tissue mass indicating a good nutritive condition likely caused a subsequent dilution of body metals leading to reduced weight-specific concentrations of some metals (Cd, Zn, Cu, Co). Sun, 01 Jan 2006 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/6674 2006-01-01T00:00:00Z https://repository.kopri.re.kr/handle/201206/7527 Title: 바지락 MT 단백질의 Cd 노출에 대한 지표 활용성 Authors: Ahn, In-Young; Ji, Jungyoun; Choi, Heeseon J. Abstract: A low-molecular weight (ca 12kD) MT-like protein (MTLP) was experimentally induced in the cytosol of R. philippinarum. Elution profiles of gel filtration chromatography showed that most of the Cd accumulated was bound to the MTLP. Immuno-histochemical analysis showed distinct antibody reactions in the epithelial cells of diverticula and of gill lamellae, which confirmed the presence of MT. The amount of Cd bound to MTLP was shown to increase with the increase of exposure time, indicating a potential utility of the MTLP as a biomarker to Cd exposure. Thu, 01 Jan 2004 00:00:00 GMT https://repository.kopri.re.kr/handle/201206/7527 2004-01-01T00:00:00Z