https://repository.kopri.re.kr/handle/201206/9794 2026-03-06T00:01:13Z 2026-03-06T00:01:13Z Cha, Ok-Kyoung Lee, Jong Eun Lee, Hyoungseok Lee, Horim https://repository.kopri.re.kr/handle/201206/10933 2022-03-24T07:14:31Z 2019-09-01T00:00:00Z

Title: Optimized protoplast isolation and establishment of transient gene expression system for the Antarctic flowering plant Colobanthus quitensis (Kunth) Bartl. Authors: Cha, Ok-Kyoung; Lee, Jong Eun; Lee, Hyoungseok; Lee, Horim Abstract: Colobanthus quitensis is one of two terrestrial plants that grow in the maritime Antarctic. Despite its important ecological niche in extreme environments, the molecular mechanisms of its adaptation and tolerance have not been elucidated due to difficulties with genetic or transgenic approaches. For this reason, in many other plant species mesophyll protoplasts as a versatile cell-based system have been developed and used to analyze the biological functions of genes of interest. Here we report an optimized method of protoplast isolation from C. quitensis leaves. The main parameters evaluated to reach the highest protoplast yield were the use of a cell wall-degrading enzyme, an osmotic stabilizer, and different pH conditions. Moreover, transient expression and subcellular localization of proteins were validated by an immunoblot assay and a confocal microscopy, respectively, using C. quitensis protoplasts. Therefore, these results suggest that protoplasts can provide a useful cell-based system to facilitate the molecular, biochemical, and cellular characterizations of C. quitensis genes.

2019-09-01T00:00:00Z Lee, Hyoungseok https://repository.kopri.re.kr/handle/201206/12008 2022-03-24T07:15:07Z 2019-05-30T00:00:00Z

Title: Establishment of Rapid Functional Verification System for Polar Plant Genetic Resources Authors: Lee, Hyoungseok

2019-05-30T00:00:00Z Byun, Mi Young Cho, Sung-Mi Lee, Jong Eun Park, Hyun Lee, Hyoungseok https://repository.kopri.re.kr/handle/201206/10901 2022-03-24T07:14:04Z 2019-01-01T00:00:00Z

Title: The complete mitochondrial genome of an Antarctic moss Chorisodontium aciphyllum (Hook. f. & Wilson) Broth Authors: Byun, Mi Young; Cho, Sung-Mi; Lee, Jong Eun; Park, Hyun; Lee, Hyoungseok Abstract: The cool-adapted species Chorisodontium aciphyllum is widespread in the maritime Antarctic. Here, we sequenced complete mitochondrial genome of C. aciphyllum (GenBank accession number:MK651511) and this is the first report on the mitogenome of the order Dicraneales. Its length is 105,766 bp and it contains 3 ribosomal RNA (rRNA), 24 transfer RNA, and 40 protein-coding genes. The mitochondrial structure and gene order was similar to other bryophytes. Phylogenetic tree based on combined analysis with amino acids sequences of 36 mitochondrial genes in C. aciphyllum, 24 Bryophyta and 3 Marchantiophyta, was well accordant with traditional species relationship of the moss.

2019-01-01T00:00:00Z