KOPRI Repository

Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321

Cited 4 time in scopus
Metadata Downloads
Title
Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321
Other Titles
남극 Streptomyces sp. AA8321 저온충격단백질A의 특성분석
Authors
Lee, Yoo Kyung
Kim, Min-Jung
Lee, Hong Kum
Im, Hana
Subject
Biochemistry & Molecular Biology
Keywords
DNA binding protein; Streptomyces; cell elongation; cold-shock protein; replication inhibitor
Issue Date
2007
Publisher
Springer
Citation
Lee, Yoo Kyung, et al. 2007. "Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321". PROTEIN JOURNAL, 26(1): 51-59.
Abstract
Polar organisms should have mechanisms to survive the extremely cold environment. Four genes encoding cold-shock proteins, which are small, cold-induced bacterial proteins, have been cloned from the Antarctic bacterium Streptomyces sp. AA8321. Since the specific functions of any polar bacterial or Streptomyces cold-shock proteins have not yet been determined, we examined the role of cold-shock protein A from Streptomyces sp. AA8321 (CspASt). Gel filtration chromatography showed that purified CspASt exists as a homodimer under physiological conditions, and gel shift assays showed that it binds to single-stranded, but not double-stranded, DNA. Overexpression of CspASt in Escherichia coli severely impaired the ability of the host cells to form colonies, and the cells developed an elongated morphology. Incorporation of a deoxynucleoside analogue, 5-bromo-2?-deoxyuridine, into newly synthesized DNA was also drastically diminished in CspASt-overexpressing cells. These results suggest that CspASt play a role in inhibition of DNA replication during cold-adaptation.
URI
http://repository.kopri.re.kr/handle/201206/5680
DOI
http://dx.doi.org/10.1007/s10930-006-9044-1
Files in This Item
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse