Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321
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- Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321
- Other Titles
- 남극 Streptomyces sp. AA8321 저온충격단백질A의 특성분석
- Lee, Yoo Kyung
Lee, Hong Kum
- Biochemistry & Molecular Biology
- DNA binding protein; Streptomyces; cell elongation; cold-shock protein; replication inhibitor
- Issue Date
- Lee, Yoo Kyung, et al. 2007. "Characterization of Cold-Shock Protein A of Antarctic Streptomyces sp. AA8321". PROTEIN JOURNAL, 26(1): 51-59.
- Polar organisms should have mechanisms to survive the extremely cold environment. Four genes encoding cold-shock proteins, which are small, cold-induced bacterial proteins, have been cloned from the Antarctic bacterium Streptomyces sp. AA8321. Since the specific functions of any polar bacterial or Streptomyces cold-shock proteins have not yet been determined, we examined the role of cold-shock protein A from Streptomyces sp. AA8321 (CspASt). Gel filtration chromatography showed that purified CspASt exists as a homodimer under physiological conditions, and gel shift assays showed that it binds to single-stranded, but not double-stranded, DNA. Overexpression of CspASt in Escherichia coli severely impaired the ability of the host cells to form colonies, and the cells developed an elongated morphology. Incorporation of a deoxynucleoside analogue, 5-bromo-2？-deoxyuridine, into newly synthesized DNA was also drastically diminished in CspASt-overexpressing cells. These results suggest that CspASt play a role in inhibition of DNA replication during cold-adaptation.
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