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Effect of the Antifreeze Protein from the Arctic Yeast Leucosporidium sp. AY30 on Cryopreservation of the Marine Diatom Phaeodactylum tricornutum

Cited 9 time in scopus
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Title
Effect of the Antifreeze Protein from the Arctic Yeast Leucosporidium sp. AY30 on Cryopreservation of the Marine Diatom Phaeodactylum tricornutum
Other Titles
북극효모 루코스포리디움 에이와이삼공 유래 결빙방지단백질의 해양규조류 피오닥틸럼 트리코누툼의 동결보존 효능
Authors
Koh, Hye Yeon
Lee, Sung Gu
Park, Hyun
Han, Se Jong
Lee, Jun Hyuck
Keywords
LeIBP; Phaeodactylum tricornutum; antifreeze protein; cryopreservation; marine diatom
Issue Date
2015
Citation
Koh, Hye Yeon, et al. 2015. "Effect of the Antifreeze Protein from the Arctic Yeast Leucosporidium sp. AY30 on Cryopreservation of the Marine Diatom Phaeodactylum tricornutum". APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 175: 677-686.
Abstract
Antifreeze proteins are a group of proteins that allow organisms to survive in subzero environments. These proteins possess thermal hysteresis and ice recrystallization inhibition activities. In the present study, we demonstrated the efficiency of a recombinant antifreeze protein from the Arctic yeast Leucosporidium sp. AY30, LeIBP, in cryopreservation of the marine diatom Phaeodactylum tricornutum, which is one of the classical model diatoms and has most widely been studied with regard to its ecology, physiology, biochemistry, and molecular biology. P. tricornutum cells were frozen by either a fast or two-step freezing method in freezing medium containing 10 % dimethyl sulfoxide, glycerol, propylene glycol, and ethylene glycol, respectively, with or without LeIBP supplement. When cells were frozen using the two-step freezing method, cell survival was significantly increased and statistically the same as that of unfrozen native cells in the presence of 0.1 mg/mL LeIBP in 10 % propylene glycol or 10 % ethylene glycol at day 11 of post-thaw culture. In the presence of LeIBP, the concentration of chlorophyll a was dramatically increased to 14-, 48-, 1.6-, and 8.8-fold when cells were frozen in freezing medium containing DMSO, glycerol, PG, and EG, respectively. Scanning electron microscopy observations demonstrated that the cells were also successfully preserved and epitheca or hypotheca were not deformed. These results demonstrate that LeIBP was successfully applied to improve cryopreservation of the marine diatom P. tricornutum.
URI
http://repository.kopri.re.kr/handle/201206/7290
DOI
http://dx.doi.org/10.1007/s12010-014-1337-9
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