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Optimization of Physical Factor for amylase Production by Arthrobacter sp.by Response Surface Methodology

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Cited 5 time in scopus
Title
Optimization of Physical Factor for amylase Production by Arthrobacter sp.by Response Surface Methodology
Other Titles
반응표면분석법을 통한 Arthrobacter sp.의 amylase 생산 최적화
Authors
Kim, Hyun-do
Han, Se Jong
Choi, Jong-il
Im, Young-kum
Keywords
AmylaseAntarctic microorganismResponse surface methodology
Issue Date
2016
Citation
Kim, Hyun-do, et al. 2016. "Optimization of Physical Factor for amylase Production by Arthrobacter sp.by Response Surface Methodology". Korean Chemical Engineering Research(HWAHAK KONGHAK), 54(1): 140-144.
Abstract
In this study, the physical factors for amylase production by Arthrobacter sp. were optimized using response surface methodology(RSM). Antarctic microorganism Arthrobacter sp. PAMC 27388 was obtained from the Polar and Alpine Microbial Collection (PAMC) at the Korea Polar Research Institute. This microorganism was confirmed for the excretion of amylase with Lugol`s solution. The amylase activity was after flask culture was as low as 1.66 mU/L before optimization. The physical factors including the inoculum volume, the initial culture pH, and the medium volume were chosen to be optimized for the enhanced amylase production. The calculated results using RSM indicate that the optimal physical factors were 2.49 mL inoculum volume, 6.85 pH and 42.87 mL medium volume with a predicted amylase production of 2.84 mU/L. The experimentally obtained amylase activity was 2.50 mU/L, which was a 150% increase compared to the level before optimization.
URI
https://repository.kopri.re.kr/handle/201206/7439
DOI
http://dx.doi.org/10.9713/kcer.2016.54.1.140
Type
Article
Indexed
KCI등재
Appears in Collections  
2014-2016, Antarctic Organisms: Cold-Adaptation Mechanism and Its Application (14-16) / Park; Hyun (PE14070; PE15070; PE16070)
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