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Enrichment of cold-active protease-producing bacteria from Arctic terrestrial and marine samples

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Title
Enrichment of cold-active protease-producing bacteria from Arctic terrestrial and marine samples
Other Titles
저온활성 단백질분해효소 분비세균의 분리
Authors
Yim, Joung Han
Cho, Kyeung Hee
Hong, Soon Gyu
Kim, Eun Hye
Cho, Jang-Cheon
Lee, Hong Kum
Keywords
enrichment; protease
Issue Date
2009
Citation
Yim, Joung Han, et al. 2009. Enrichment of cold-active protease-producing bacteria from Arctic terrestrial and marine samples. 한국미생물학회. 한국미생물학회. 2009.05.29~.
Abstract
Protease-producing bacterial strains were isolated by enrichment in 0.1X NA or ZoBell semi-solid agar medium supplemented with pork pieces at 4
C for 6 months. Thirty eight and 51 strains were isolated from Arctic terrestrial and marine samples, respectively. Optimal temperature for growth ranged between 4
C and 30
C depending on the strains. Protease secretion was observed in 48 strains, forming hydrolytic zone on solid media supplemented with skim milk. They were included in Pseudoalteromonas (33 strains), Pseudomonas (10 strains), Arthrobacter (4 strains), and Flavobacterium (1 strain). Protease secretion was higher at 4
C than at 20
C in most strains. From these results, it is concluded that bacterial strains producing cold-active protease could be effectively enriched by long-term incubation at 4
C in semi-solid agar medium.
URI
http://repository.kopri.re.kr/handle/201206/7909
Conference Name
한국미생물학회
Conference Place
한국미생물학회
Conference Date
2009.05.29~
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