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Effect of carbon sources on the fed-batch production of prodigiosin by Hahella chejuensis M3349

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Title
Effect of carbon sources on the fed-batch production of prodigiosin by Hahella chejuensis M3349
Other Titles
Hahella chejuensis M3349에 의한 프로디지오신의 유가식 생산에서 탄소원의 영향
Authors
Yim, Joung Han
Lee, Hong Kum
Park, Heeyong
Lee, Sung Gu
Han, Se Jong
Keywords
Algicidal activity; Fed-batch; Prodigiosin; Red-tide
Issue Date
2010
Citation
Yim, Joung Han, et al. 2010. Effect of carbon sources on the fed-batch production of prodigiosin by Hahella chejuensis M3349. 한국생물공학회. 한국생물공학회. 2010.04.16~.
Abstract
In order to increase prodigiosin production from Hahella chejuensis M3349, fed-batch cultures using various carbon sources were performed in 5L jar fermentor for 6 days. Cells were subjected to prodigiosin extraction using 1.2 vol. ethanol of culture broth. Concentration of prodigiosin extract was diluted to 10ppm and then algaecidal activity of diluted prodigiosin was measured against Cochlodinium polykrikodes which leads to red tides. Ultimately, prodigiosin production of 1.34, 0.77 and 2.35g/L was obtained and algaecidal activity of 93.05±2.3, 95.22±2.3 and 98.14±1.53% was confirmed when fed-batch culture was performed with sucrose, glycerol and glucose, respectively. This work shows that glucose was the best candidate for enhanced algaecidal activity and productivity of prodigiosin from Hahella chejuensis M3349 by fed-batch. [This work was supported by a grant from the Korea Polar Research Institute (PE10050 and PK09040).]
URI
http://repository.kopri.re.kr/handle/201206/8009
Conference Name
한국생물공학회
Conference Place
한국생물공학회
Conference Date
2010.04.16~
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