Effect of carbon sources on the fed-batch production of prodigiosin by Hahella chejuensis M3349

Abstract

In order to increase prodigiosin production from Hahella chejuensis M3349, fed-batch cultures using various carbon sources were performed in 5L jar fermentor for 6 days. Cells were subjected to prodigiosin extraction using 1.2 vol. ethanol of culture broth. Concentration of prodigiosin extract was diluted to 10ppm and then algaecidal activity of diluted prodigiosin was measured against Cochlodinium polykrikodes which leads to red tides. Ultimately, prodigiosin production of 1.34, 0.77 and 2.35g/L was obtained and algaecidal activity of 93.05±2.3, 95.22±2.3 and 98.14±1.53% was confirmed when fed-batch culture was performed with sucrose, glycerol and glucose, respectively. This work shows that glucose was the best candidate for enhanced algaecidal activity and productivity of prodigiosin from Hahella chejuensis M3349 by fed-batch. [This work was supported by a grant from the Korea Polar Research Institute (PE10050 and PK09040).]