KOPRI Repository

Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization

Metadata Downloads
Title
Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization
Other Titles
남북극 미생물 Culture Collection으로부터 저온활성 단백질분해효소 생산 균주 선발 및 효소의 특성연구
Authors
Park, Ha Ju
Yim, Joung Han
Lee, Yung Mi
Lee, Hong Kum
Hong, Soon Gyu
Kim, Dockyu
Keywords
Cold-active; Culture Collection of Polar Mi; Protease; Screening
Issue Date
2010
Citation
Park, Ha Ju, et al. 2010. Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization. 한국미생물학회. 한국미생물학회. 2010.05.06~.
Abstract
KOPRI has assembled a culture collection of cold-adapted bacteria from the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected according to their relative activities and were subsequently re-examined for their extracellular protease activity on ZoBell/skim milk plates. This screening method permitted the selection of a small group of 15 cold-adapted bacteria, belonging to the genus Pseudoalteromonas or Flavobacterium, that showed proteolytic activities at 5–
15°C. The cold-active proteases from these strains were classified into four categories according to the extent of enzymatic inhibition by a class-specific protease inhibitor. Since highly active and cold-adapted proteases have the potential for commercial enzyme development, the bacteria selected in this work are studied as a valuable natural source of new proteases. At present, a protease from one strain (KOPRI 21717), which is highly active at low temperatures and resistant to protein-denaturing SDS, is being studied for future application in detergent.
URI
http://repository.kopri.re.kr/handle/201206/8074
Conference Name
한국미생물학회
Conference Place
한국미생물학회
Conference Date
2010.05.06~
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    qrcode

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse