Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization
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- Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization
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- 남북극 미생물 Culture Collection으로부터 저온활성 단백질분해효소 생산 균주 선발 및 효소의 특성연구
- Park, Ha Ju
Yim, Joung Han
Lee, Yung Mi
Lee, Hong Kum
Hong, Soon Gyu
- Cold-active; Culture Collection of Polar Mi; Protease; Screening
- Issue Date
- Park, Ha Ju, et al. 2010. Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization. 한국미생물학회. 한국미생물학회. 2010.05.06~.
- KOPRI has assembled a culture collection of cold-adapted bacteria from the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected according to their relative activities and were subsequently re-examined for their extracellular protease activity on ZoBell/skim milk plates. This screening method permitted the selection of a small group of 15 cold-adapted bacteria, belonging to the genus Pseudoalteromonas or Flavobacterium, that showed proteolytic activities at 5–
15°C. The cold-active proteases from these strains were classified into four categories according to the extent of enzymatic inhibition by a class-specific protease inhibitor. Since highly active and cold-adapted proteases have the potential for commercial enzyme development, the bacteria selected in this work are studied as a valuable natural source of new proteases. At present, a protease from one strain (KOPRI 21717), which is highly active at low temperatures and resistant to protein-denaturing SDS, is being studied for future application in detergent.
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