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Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization

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dc.contributor.authorPark, Ha Ju-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorLee, Yung Mi-
dc.contributor.authorLee, Hong Kum-
dc.contributor.authorHong, Soon Gyu-
dc.contributor.authorKim, Dockyu-
dc.date.issued2010-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/8074-
dc.description.abstractKOPRI has assembled a culture collection of cold-adapted bacteria from the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected according to their relative activities and were subsequently re-examined for their extracellular protease activity on ZoBell/skim milk plates. This screening method permitted the selection of a small group of 15 cold-adapted bacteria, belonging to the genus Pseudoalteromonas or Flavobacterium, that showed proteolytic activities at 5&#8211-
dc.description.abstract15°C. The cold-active proteases from these strains were classified into four categories according to the extent of enzymatic inhibition by a class-specific protease inhibitor. Since highly active and cold-adapted proteases have the potential for commercial enzyme development, the bacteria selected in this work are studied as a valuable natural source of new proteases. At present, a protease from one strain (KOPRI 21717), which is highly active at low temperatures and resistant to protein-denaturing SDS, is being studied for future application in detergent.-
dc.languageEnglish-
dc.titleScreening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization-
dc.title.alternative남북극 미생물 Culture Collection으로부터 저온활성 단백질분해효소 생산 균주 선발 및 효소의 특성연구-
dc.typeProceeding-
dc.identifier.bibliographicCitationPark, Ha Ju, et al. 2010. Screening for Cold-active Protease-producing Bacteria from the Culture Collection of Polar Microorganisms and Protease Characterization. 한국미생물학회. 한국미생물학회. 2010.05.06~.-
dc.citation.volume1-
dc.citation.number1-
dc.citation.conferenceDate2010.05.06~-
dc.citation.conferenceName한국미생물학회-
dc.citation.conferencePlace한국미생물학회-
dc.description.articleClassificationPro(초록)국내-
dc.subject.keywordCold-active-
dc.subject.keywordCulture Collection of Polar Mi-
dc.subject.keywordProtease-
dc.subject.keywordScreening-
dc.identifier.localId2010-0192-
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han
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