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Cloning in Escherichia coli of Hahella chejuensis Genes Encoding Prodigiosin Biosynthesis

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Title
Cloning in Escherichia coli of Hahella chejuensis Genes Encoding Prodigiosin Biosynthesis
Other Titles
Hahella chejuensis 프로디지오신 생산 관련 유전자의 클로닝
Authors
안주용
Lee, Yoo Kyung
Lee, Hong Kum
Kim, Il-Chan
Keywords
Hahella chejuensis; MAP; MBC; Serratia; prodigiosin
Issue Date
2006
Citation
안주용, et al. 2006. Cloning in Escherichia coli of Hahella chejuensis Genes Encoding Prodigiosin Biosynthesis. 한국미생물학회. 한국미생물학회. 2006.05.03~.
Abstract
Hahella chejuensis is a novel marine bacterium that produces the red pigment, prodigiosin. The pathway for the biosynthesis of prodigiosin in some Serratia strains has been recently suggested as a furcated pathway that terminates in the enzymatic condensation of the two final products, 4-methoxy-2,2-bipprrole-5-carboxyaldehyde (MBC) and 2-methyl-3-n-amyl-pyrrole(MAP). Full genome sequence analysis of H. chejuensis revealed important similarities between the Serratia prodigiosin biosynthesis gene cluster and several ORFs in H. chejuensis. The respective partial or whole sequences for MAP biosynthesis among those ORFs from H. chejuensis were cloned and expressed in Escherichia coli. Some clones produced the red pigment on the LB agar plate in the presence of MBC. The putative prodigiosin biosynthesis gene cluster and a scheme for the biosynthetic pathway of H. chejuensis are proposed in this study.
URI
http://repository.kopri.re.kr/handle/201206/8082
Conference Name
한국미생물학회
Conference Place
한국미생물학회
Conference Date
2006.05.03~
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