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Enhanced Production ofEndochitinase from An Antarctic Bacterium,Sanguibacter antarcticus PAMC21702, in Pichia pastoris

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Title
Enhanced Production ofEndochitinase from An Antarctic Bacterium,Sanguibacter antarcticus PAMC21702, in Pichia pastoris
Other Titles
피키아 패스토리스에서 남극세균 상귀박터 안탁티쿠스 PAMC 21702 유래 키틴분해효소의 생산성 증대
Authors
Han, Se Jong
Yim, Joung Han
Koh, Hye Yeon
Park, Heeyong
Lee, Sung Gu
Keywords
Antarctica; Cold-active; Endochitinases; Fed-batch; Pichia pastoris
Issue Date
2014
Citation
Han, Se Jong, et al. 2014. "Enhanced Production ofEndochitinase from An Antarctic Bacterium,Sanguibacter antarcticus PAMC21702, in Pichia pastoris". JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 8(5): 3531-3536.
Abstract
The recombinant endochitinase (rChi21702) from a psychrophilic Antarctic bacterium, Sanguibacter antarcticus (KCTC 13143, PAMC 21702), has been produced using Pichia pastoris expression system. In this study, the optimum fermentation temperature (20°C) and growth media pH (6) for Chi21702 production was determined using a 2-stage fed-batch culture system. The maximal enzyme activity (178 U/l) of rChi21702 obtained using a glycerol-methanol fed-batch culture system was approximately 6-fold higher than that of unoptimized conditions. In addition, the rChi21702 exhibited cold-active endochitinase activity and higher thermal stability than that of the wild-type Chi21702. This work allows for large-scale production of rChi21702, which could be extended to further application studies using recombinant chtinases.
URI
http://repository.kopri.re.kr/handle/201206/6612
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