Enhanced Production ofEndochitinase from An Antarctic Bacterium,Sanguibacter antarcticus PAMC21702, in Pichia pastoris

Abstract

The recombinant endochitinase (rChi21702) from a psychrophilic Antarctic bacterium, Sanguibacter antarcticus (KCTC 13143, PAMC 21702), has been produced using Pichia pastoris expression system. In this study, the optimum fermentation temperature (20°C) and growth media pH (6) for Chi21702 production was determined using a 2-stage fed-batch culture system. The maximal enzyme activity (178 U/l) of rChi21702 obtained using a glycerol-methanol fed-batch culture system was approximately 6-fold higher than that of unoptimized conditions. In addition, the rChi21702 exhibited cold-active endochitinase activity and higher thermal stability than that of the wild-type Chi21702. This work allows for large-scale production of rChi21702, which could be extended to further application studies using recombinant chtinases.