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Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17

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Title
Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17
Other Titles
Rhodococcus sp. strain DK17 유래 o-xylene dioxygenase에 의한 biphenyl hydroxylation 반응
Authors
Kim, Eungbin
Kang, Beom Sik
Kim, Dockyu
Yoo, Miyoun
Keywords
Rhodococcus; biphenyl; hydoxylation; mutant; o-xylene dioxygenase
Issue Date
2011
Citation
Kim, Eungbin, et al. 2011. Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17. 한국미생물학회. 한국미생물학회. 2011.05.12~.
Abstract
Hydroxylation of the nongrowth substrate biphenyl by recombinant o-xylene dioxygenases from Rhodococcus sp. strain DK17 was studied through bioconversion experiments. The metabolites from the biphenyl hydroxylation by each enzyme were identified and quantified by gas chromatography-mass spectrometry. The L266F mutant enzyme produced much more 2 hydroxybiphenyl (2.43 vs. 0.1 μg/L) and 3 hydroxybiphenyl (1.97 vs. 0.03 μ g/L) than the wild-type. Site-directed mutagenesis combined with structural and functional analyses indicated that hydrophobic interactions and shielding effects against water are important factors in the hydroxylation of biphenyl by the o xylene dioxygenase. The residue at position 266 plays a key role in coordinating the reaction.
URI
http://repository.kopri.re.kr/handle/201206/8506
Conference Name
한국미생물학회
Conference Place
한국미생물학회
Conference Date
2011.05.12~
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