Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17
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- Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17
- Other Titles
- Rhodococcus sp. strain DK17 유래 o-xylene dioxygenase에 의한 biphenyl hydroxylation 반응
- Kim, Eungbin
Kang, Beom Sik
- Rhodococcus; biphenyl; hydoxylation; mutant; o-xylene dioxygenase
- Issue Date
- Kim, Eungbin, et al. 2011. Biphenyl hydroxylation enhanced by an engineered o-xylene dioxygenase from Rhodococcus sp. strain DK17. 한국미생물학회. 한국미생물학회. 2011.05.12~.
- Hydroxylation of the nongrowth substrate biphenyl by recombinant o-xylene dioxygenases
from Rhodococcus sp. strain DK17 was studied through bioconversion experiments. The
metabolites from the biphenyl hydroxylation by each enzyme were identified and
quantified by gas chromatography-mass spectrometry. The L266F mutant enzyme produced
much more 2 hydroxybiphenyl (2.43 vs. 0.1 μg/L) and 3 hydroxybiphenyl (1.97 vs. 0.03 μ
g/L) than the wild-type. Site-directed mutagenesis combined with structural and
functional analyses indicated that hydrophobic interactions and shielding effects
against water are important factors in the hydroxylation of biphenyl by the o xylene
dioxygenase. The residue at position 266 plays a key role in coordinating the reaction.
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