Enhancing Lipid Production in the Arctic Microalga Chlamydomonas sp. KNM0029C by Using Statistical Optimization of Medium Components

Abstract

A sequential statistical optimization was applied tothe components of Tris-acetate-phosphate (TAP) medium, toenhance the cell density and lipid production of Arcticmicroalga Chlamydomonas sp. KNM0029C. The evaluationwas preformed through an elimination process and Plackett-Burman design to select for significant factors, contributingtoward cell growth. NH4Cl, MgSO4·7H2O, potassium phosphate,and trace elements were established as the effectivecomponents and used as variable factors in Box-Behnken designtest, - a response surface methodology (RSM). The final optimizedconcentrations were 0.54, 0.16, 0.04, and 0.08 g/L ofNH4Cl, MgSO4·7H2O, potassium phosphate, and trace elements,respectively. Overall, 9.9% enhanced lipid productionwas achieved by using optimized TAP medium at 4°C. Theresults of the present study could potentially contributetoward large-scale lipid production at low temperatures.