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Crystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14

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dc.contributor.authorLee, Chang Woo-
dc.contributor.authorPark, Sun-Ha-
dc.contributor.authorKoh, Hye Yeon-
dc.contributor.authorJeong, Chang Sook-
dc.contributor.authorHwang, Jisub-
dc.contributor.authorLee, Sung Gu-
dc.contributor.authorYoun, Ui Joung-
dc.contributor.authorLee, Chang Sup-
dc.contributor.authorPark, Hyun Ho-
dc.contributor.authorKim, Hak Jun-
dc.contributor.authorPark, Hyun-
dc.contributor.authorLee, Jun Hyuck-
dc.date.accessioned2020-10-20T04:45:26Z-
dc.date.available2020-10-20T04:45:26Z-
dc.date.issued2019-05-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/10895-
dc.description.abstractIn cold and harsh environments such as glaciers and sediments in ice cores, microbes can survive by forming spores. Spores are composed of a thick coat protein, which protects against external factors such as heat-shock, high salinity, and nutrient deficiency. GerE is a key transcription factor involved in spore coat protein expression in the mother cell during sporulation. GerE regulates transcription during the late sporulation stage by directly binding to the promoter of cotB gene. Here, we report the crystal structure of PaGerE at 2.09 A resolution from Paenisporosarcina sp. TG-14, which was isolated from the Taylor glacier. The PaGerE structure is composed of four α-helices and adopts a helix-turn-helix architecture with 68 amino acid residues. Based on our DNA binding analysis, the PaGerE binds to the promoter region of CotB to affect protein expression. Additionally, our structural comparison studies suggest that DNA binding by PaGerE causes a conformational change in the α4-helix region, which may strongly induce dimerization of PaGerE.en_US
dc.languageEnglishen_US
dc.language.isoenen_US
dc.subjectBiochemistry & Molecular Biologyen_US
dc.subjectBiophysicsen_US
dc.subject.classificationKing Sejong Stationen_US
dc.titleCrystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14en_US
dc.title.alternative스포어를 형성하는 빙하미생물 (Paenisporosarcina sp. TG-14) 유래 PaGerE 단백질의 구조연구en_US
dc.typeArticleen_US
dc.identifier.bibliographicCitationLee, Chang Woo, et al. 2019. "Crystal structure of a transcription factor, GerE (PaGerE), from spore-forming bacterium Paenisporosarcina sp. TG-14". <em>BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS</em>, 513(2): 374-379.-
dc.citation.titleBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONSen_US
dc.citation.volume513en_US
dc.citation.number2en_US
dc.identifier.doi10.1016/j.bbrc.2019.04.019-
dc.citation.startPage374en_US
dc.citation.endPage379en_US
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2017:48.611en_US
dc.subject.keywordGerEen_US
dc.subject.keywordPaenisporosarcina sp. TG-14en_US
dc.subject.keywordX-ray crystallographyen_US
dc.subject.keywordsporeen_US
dc.identifier.localId2019-0052-
dc.identifier.scopusid2-s2.0-85064825948-
dc.identifier.wosid000468258600013-
Appears in Collections  
2019-2019, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (19-19) / Kim, Jin-Hyoung (PE19080)
2019-2019, Development of potential candidates as antibiotics based on polar genetic resources (19-19) / Lee, Jun Hyuck (PE19210)
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