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Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model

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dc.contributor.authorHong, Ju-Mi-
dc.contributor.authorKim, Jung Eun-
dc.contributor.authorMin, Seul Ki-
dc.contributor.authorKim, Kyung Hee-
dc.contributor.authorHan, Se Jong-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorPark, Hyun-
dc.contributor.authorKim, Jin-Hyoung-
dc.contributor.authorKim, Il-Chan-
dc.date.accessioned2021-05-11T04:35:56Z-
dc.date.available2021-05-11T04:35:56Z-
dc.date.issued2021-02-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/11953-
dc.description.abstractUmbilicaria antarctica (UA) is a member of the family Umbilicariaceae. To the best of our knowledge, no studies on its anti-inflammatory effects have been reported yet. In the present study, we examined its ability to suppress inflammatory responses and the molecular mechanisms underlying these abilities using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and a zebrafish model of inflammation. We investigated the effects of UA on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW 264.7 cells. To explore the anti-inflammatory mechanisms of UA, we measured the mRNA and protein expression of pro-inflammatory mediators in LPS-stimulated RAW 264.7 cells using quantitative RT-PCR and western blot analyses, respectively. UA significantly inhibited the production of NO, PGE2, interleukin (IL)-6, and tumor necrosis factor (TNF)-α in the LPS-stimulated RAW 264.7 cells. It also suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor (NF)-κB activation in LPS-stimulated RAW 264.7 cells and tail pin-cutting induced zebrafish model. Collectively, these findings indicate that UA significantly inhibits LPS-stimulated inflammatory responses. These effects were considered to be strongly associated with the suppression of NF-κB activation. Overall, our results demonstrate that UA extract exerts strong anti-inflammatory activities in in vitro and in vivo models and suggest that UA may be an effective novel therapeutic agent for the treatment of inflammatory diseases.en_US
dc.languageEnglishen_US
dc.language.isoen_USen_US
dc.subjectBiotechnology & Applied Microbiologyen_US
dc.subjectResearch & Experimental Medicineen_US
dc.subject.classificationKing Sejong Stationen_US
dc.titleAnti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Modelen_US
dc.title.alternative남극 지의류 Umbilicaria Antarctica 추출물이 RAW264.7 세포와 zebrafish 모델에서의 항염증 효능en_US
dc.typeArticleen_US
dc.identifier.bibliographicCitationHong, Ju-Mi, et al. 2021. "Anti-Inflammatory Effects of Antarctic Lichen Umbilicaria antarctica Methanol Extract in Lipopolysaccharide-Stimulated RAW 264.7 Macrophage Cells and Zebrafish Model". <em>BIOMED RESEARCH INTERNATIONAL</em>, 2021(1): 1-12.en_US
dc.citation.titleBIOMED RESEARCH INTERNATIONALen_US
dc.citation.volume2021en_US
dc.citation.number1en_US
dc.identifier.doi10.1155/2021/8812090-
dc.citation.startPage1en_US
dc.citation.endPage12en_US
dc.description.articleClassificationSCIE-
dc.description.jcrRateJCR 2019:58.974en_US
dc.subject.keywordAntarctic lichenen_US
dc.subject.keywordAnti-inflammationen_US
dc.subject.keywordUmbilicaria Antarcticaen_US
dc.identifier.localId2021-0025-
dc.identifier.scopusid2-s2.0-85102054346-
dc.identifier.wosid000625188200007-
Appears in Collections  
2021-2021, Commercialization of new Biomaterials from polar organisms (21-21) / Yim, Joung Han (PE21150)
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