Enzymatic synthesis of anabolic steroids glycosides by glucosyltransferase from Terribacillus sp. PAMC 23288

Abstract

To date, the use of steroid drugs has been steadily thanks to its effects, but due to severe side effects, studies on the derivative activity of steroids are being conducted. Nandrolone, a breast cancer drug, cortisone and prednisone which reduce inflammation, pain, and edema used in this study are not known as sugar derivatives. O-glucose was successfully linked using UDP-glucosyltransferase (UGT-1), and bioactivity was tested in vitro. NMR analysis found that those products produced by the nandrolone and testosterone were 17β-nandrolone β-D-glucose and 17β-testosterone β-D-glucose, respectively. In addition, testosterone derivatives in rotenone-treated PC12 cells showed higher cell viability and no cytotoxicity compared to control. In the presence of H2O2 in SH-SY5Y cells, nandrolone derivatives showed cell viability and attenuated cytotoxicity. Both derivatives also increased the renal protective effect and cellular ATP.