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MALDI-MS-Based Quantitative Analysis for Ketone Containing Homoserine Lactones in Pseudomonas aeruginosa

Cited 27 time in wos
Cited 28 time in scopus

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dc.contributor.authorKim, Yoon-Woo-
dc.contributor.authorSung, Changmin-
dc.contributor.authorLee, Seulee-
dc.contributor.authorKim, Kyoung-Jin-
dc.contributor.authorYang, Yung-Hun-
dc.contributor.authorKim, Byung-Gee-
dc.contributor.authorLee, Yoo Kyung-
dc.contributor.authorRyu, Hee Wook-
dc.contributor.authorKim, Yun-Gon-
dc.date.accessioned2021-11-29T05:04:30Z-
dc.date.available2021-11-29T05:04:30Z-
dc.date.issued2015-01-20-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/13019-
dc.description.abstractN-acyl homoserine lactones (AHLs), quorum sensing molecules produced by gram-negative bacteria, are used as important secondary metabolites for antibacterial drug development and cell-to-cell communication. Although various analytical techniques have been developed for detection and quantitation of AHLs from more complex bacterial culture media, only a few methods have been applied to AHL identification in physiological samples. Here, we developed a highly sensitive and reliable MALDI-based 3-oxo AHL quantitation method by employing Girard’s reagent T (GT) to produce a permanent cationic charge state [M]+ at the ketone group of AHLs. After extracting AHLs from the supernatant of bacterial cultures using ethyl acetate, the extracts were subsequently derivatized with GT without any additional purification or desalting steps. The chemical derivatization of 3-oxo AHLs dramatically enhanced sensitivity (up to 60,000 times) by lowering the limit of detection (LOD, ~ 0.5 fmol)/limit of quantitation (LOQ, ~ 2.5 fmol). Additionally, the GT-derivatized 3-oxo AHLs allowed more accurate quantitative analysis from the Pseudomonas aeruginosa PAO1 culture supernatants. This method may be applied for developing high-throughput and sensitive detection methods of quorum sensing signal molecules in biofilm-related clinical applications such as virulence factor characterization and antibacterial drug development.en_US
dc.languageEnglishen_US
dc.language.isoenen_US
dc.subjectChemistryen_US
dc.titleMALDI-MS-Based Quantitative Analysis for Ketone Containing Homoserine Lactones in Pseudomonas aeruginosaen_US
dc.title.alternativePseudomonas aeruginosa의 ketone containinghomoserine lactones MALDI-MS-기반 정량분석법en_US
dc.typeArticleen_US
dc.identifier.bibliographicCitationKim, Yoon-Woo, et al. 2015. "MALDI-MS-Based Quantitative Analysis for Ketone Containing Homoserine Lactones in Pseudomonas aeruginosa". <em>ANALYTICAL CHEMISTRY</em>, 87(2): 858-863.-
dc.citation.titleANALYTICAL CHEMISTRYen_US
dc.citation.volume87en_US
dc.citation.number2en_US
dc.identifier.doi10.1021/ac5039362-
dc.citation.startPage858en_US
dc.citation.endPage863en_US
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2013:5.263157894736842en_US
dc.subject.keywordN-ACYLHOMOSERINE LACTONESen_US
dc.subject.keywordCHROMATOGRAPHY-MASS SPECTROMETRYen_US
dc.subject.keywordASSISTED-LASER-DESORPTION/IONIZATIONen_US
dc.subject.keywordSENSING SIGNAL MOLECULESen_US
dc.subject.keywordBACTERIAL BIOFILMSen_US
dc.subject.keywordCYSTIC-FIBROSISen_US
dc.subject.keywordQUORUMen_US
dc.subject.keywordQUANTIFICATIONen_US
dc.subject.keywordVIRULENCEen_US
dc.subject.keywordPERFORMANCEen_US
dc.identifier.localId2015-0008-
dc.identifier.scopusid2-s2.0-84922311563-
dc.identifier.wosid000348332300006-
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