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Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice

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dc.contributor.authorByun, Mi Young-
dc.contributor.authorCui, Li Hua-
dc.contributor.authorLee, Andosung-
dc.contributor.authorOh, Hyung Geun-
dc.contributor.authorYoo, Yo-Han-
dc.contributor.authorLee, Jungeun-
dc.contributor.authorKim, Woo Taek-
dc.contributor.authorLee, Hyoungseok-
dc.description.abstractThe Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance that allow D. antarctica to colonize harsh Antarctic environments; however, the molecular mechanisms of its responses to environmental changes remain poorly understood. To elucidate the survival strategies of D. antarctica in Antarctic environments, we investigated the functions of actin depolymerizing factor (ADF) in this species. We identified eight ADF genes in the transcriptome that were clustered into five subgroups by phylogenetic analysis. DaADF3, which belongs to a monocot-specific clade together with cold-responsive ADF in wheat, showed significant transcriptional induction in response to dehydration and cold, as well as under Antarctic field conditions. Multiple drought and low-temperature responsive elements were identified as possible binding sites of C-repeat-binding factors in the promoter region of DaADF3, indicating a close relationship between DaADF3 transcription control and abiotic stress responses. To investigate the functions of DaADF3 related to abiotic stresses in vivo, we generated transgenic rice plants overexpressing DaADF3. These transgenic plants showed greater tolerance to low-temperature stress than the wild-type in terms of survival rate, leaf chlorophyll content, and electrolyte leakage, accompanied by changes in actin filament organization in the root tips. Together, our results imply that DaADF3 played an important role in the enhancement of cold tolerance in transgenic rice plants and in the adaptation of D. antarctica to its extreme environment.en_US
dc.subject.classificationKing Sejong Stationen_US
dc.titleAbiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Riceen_US
dc.title.alternative남극좀새풀 DaADF3 유전자 과발현을 통한 벼 내냉성 증대en_US
dc.identifier.bibliographicCitationByun, Mi Young, et al. 2021. "Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice". <em>FRONTIERS IN PLANT SCIENCE</em>, 12: 1-13.-
dc.description.jcrRateJCR 2019:8.12en_US
dc.subject.keywordabiotic stressen_US
dc.subject.keywordactin cytoskeletonen_US
dc.subject.keywordDeschampsia antarctica actin-depolymerizing factor 3en_US
dc.subject.keywordlow temperatureen_US
dc.subject.keywordpolar adaptationen_US
Appears in Collections  
2021-2021, Post-Polar Genomics Project: Functional genomic study for securing of polar useful genes (21-21) / Kim, Jin-Hyoung (PE21160)
2021-2021, Development of potential candidates as antibiotics based on polar genetic resources (21-21) / Lee, Jun Hyuck (PM21030)
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