Investigating the Functional Role of the Cysteine Residue in Dehydrin from the Arctic Mouse-Ear Chickweed Cerastium arcticum
DC Field | Value | Language |
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dc.contributor.author | 김일섭 | - |
dc.contributor.author | Choi, Woong | - |
dc.contributor.author | Park, Ae Kyung | - |
dc.contributor.author | Kim, Hyun | - |
dc.contributor.author | 손종현 | - |
dc.contributor.author | Lee, Jun Hyuck | - |
dc.contributor.author | Shin, Seung Chul | - |
dc.contributor.author | 김두헌 | - |
dc.contributor.author | Kim, Han-Woo | - |
dc.date.accessioned | 2022-10-04T16:36:41Z | - |
dc.date.available | 2022-10-04T16:36:41Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | https://repository.kopri.re.kr/handle/201206/13921 | - |
dc.description.abstract | The stress-responsive, SK5 subclass, dehydrin gene, CaDHN, has been identified from the Arctic mouse-ear chickweed Cerastium arcticum. CaDHN contains an unusual single cysteine residue (Cys143), which can form intermolecular disulfide bonds. Mutational analysis and a redox experiment confirmed that the dimerization of CaDHN was the result of an intermolecular disulfide bond between the cysteine residues. The biochemical and physiological functions of the mutant C143A were also investigated by in vitro and in vivo assays using yeast cells, where it enhanced the scavenging of reactive oxygen species (ROS) by neutralizing hydrogen peroxide. Our results show that the cysteine residue in CaDHN helps to enhance C. arcticum tolerance to abiotic stress by regulating the dimerization of the intrinsically disordered CaDHN protein, which acts as a defense mechanism against extreme polar environments. | - |
dc.language | English | - |
dc.subject.classification | Dasan Station | - |
dc.title | Investigating the Functional Role of the Cysteine Residue in Dehydrin from the Arctic Mouse-Ear Chickweed Cerastium arcticum | - |
dc.title.alternative | 북극점나도나물 유래 디하이드린 단백질에서의 시스테인 잔기의 기능성에 관한 연구 | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | 김일섭, et al. 2022. "Investigating the Functional Role of the Cysteine Residue in Dehydrin from the Arctic Mouse-Ear Chickweed Cerastium arcticum". <em>MOLECULES</em>, 27(9): 1-9. | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 27 | - |
dc.citation.number | 9 | - |
dc.identifier.doi | 10.3390/molecules27092934 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.description.articleClassification | SCIE | - |
dc.description.jcrRate | JCR 2020:35.393 | - |
dc.subject.keyword | Arctic mouse-ear chickweed | - |
dc.subject.keyword | cysteine | - |
dc.subject.keyword | dehydrin | - |
dc.subject.keyword | dimerization | - |
dc.subject.keyword | intermolecular disulfide bond | - |
dc.subject.keyword | reactive oxygen species | - |
dc.identifier.localId | 2022-0075 | - |
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