Feruloyl Esterase (LaFae) from Lactobacillus acidophilus: Structural Insights and Functional Characterization for Application in Ferulic Acid Production
DC Field | Value | Language |
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dc.contributor.author | Sangeun Jeon | - |
dc.contributor.author | Hwang, Jisub | - |
dc.contributor.author | Do, Hackwon | - |
dc.contributor.author | Ly Thi Huong Luu Le | - |
dc.contributor.author | Lee, Chang Woo | - |
dc.contributor.author | Wanki Yoo | - |
dc.contributor.author | 이민주 | - |
dc.contributor.author | Shin, Seung Chul | - |
dc.contributor.author | Kyeong Kyu Kim | - |
dc.contributor.author | Kim, Han-Woo | - |
dc.contributor.author | Lee, Jun Hyuck | - |
dc.date.accessioned | 2023-12-06T16:38:16Z | - |
dc.date.available | 2023-12-06T16:38:16Z | - |
dc.date.issued | 2023 | - |
dc.identifier.uri | https://repository.kopri.re.kr/handle/201206/14891 | - |
dc.description.abstract | Ferulic acid and related hydroxycinnamic acids, used as antioxidants and preservatives in the food, cosmetic, pharmaceutical and biotechnology industries, are among the most abun-dant phenolic compounds present in plant biomass. Identification of novel compounds that can produce ferulic acid and hydroxycinnamic acids, that are safe and can be mass-produced, is criti-cal for the sustainability of these industries. In this study, we aimed to obtain and characterize a feruloyl esterase (LaFae) from Lactobacillus acidophilus. Our results demonstrated that LaFae reacts with ethyl ferulate and can be used to effectively produce ferulic acid from wheat bran, rice bran and corn stalks. In addition, xylanase supplementation was found to enhance LaFae enzymatic hydrolysis, thereby augmenting ferulic acid production. To further investigate the active site configuration of LaFae, crystal structures of unliganded and ethyl ferulate-bound LaFae were determined at 2.3 and 2.19 A resolutions, respectively. Structural analysis shows that a Phe34 residue, located at the active site entrance, acts as a gatekeeper residue and controls substrate binding. Mutating this Phe34 to Ala produced an approximately 1.6-fold increase in LaFae activ-ity against p-nitrophenyl butyrate. Our results highlight the considerable application potential of LaFae to produce ferulic acid from plant biomass and agricultural by-products. | - |
dc.language | English | - |
dc.subject.classification | 해당사항없음 | - |
dc.title | Feruloyl Esterase (LaFae) from Lactobacillus acidophilus: Structural Insights and Functional Characterization for Application in Ferulic Acid Production | - |
dc.title.alternative | 혐기성 미생물 (Lactobacillus acidophilus) 유래의 Feruloyl Esterase 효소의 구조와 기능 규명을 통한 Ferulic Acid 생산에 활용 | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Sangeun Jeon, et al. 2023. "Feruloyl Esterase (LaFae) from Lactobacillus acidophilus: Structural Insights and Functional Characterization for Application in Ferulic Acid Production". <em>INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES</em>, 24(13): 1-17. | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 24 | - |
dc.citation.number | 13 | - |
dc.identifier.doi | 10.3390/ijms241311170 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 17 | - |
dc.description.articleClassification | SCIE | - |
dc.description.jcrRate | JCR 2021:23.232 | - |
dc.subject.keyword | LaFae | - |
dc.subject.keyword | X-ray crystallography | - |
dc.subject.keyword | crystal structure | - |
dc.subject.keyword | ferulic acid | - |
dc.subject.keyword | feruloyl esterase | - |
dc.identifier.localId | 2023-0112 | - |
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