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RNA outperforms DNA-based metabarcoding in assessing the diversity and response of microeukaryotes to environmental variables in the Arctic Ocean

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dc.contributor.authorKong Hejun-
dc.contributor.authorYang, Eun Jin-
dc.contributor.authorJiao Nianzhi-
dc.contributor.authorLee, Youngju-
dc.contributor.authorJung, Jinyoung-
dc.contributor.authorCho, Kyoung-Ho-
dc.contributor.authorMoon, Jung Kuk-
dc.contributor.author김지훈-
dc.contributor.authorXu Dapeng-
dc.date.accessioned2023-12-06T16:38:35Z-
dc.date.available2023-12-06T16:38:35Z-
dc.date.issued2023-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/14913-
dc.description.abstractThe Arctic Ocean (AO) has a harsh environment characterized by low temperatures, extensive ice coverage, and periodic freezing and melting of sea ice, which has provided diverse habitats for microorganisms. Prior studies primarily focused on microeukaryote communities in the upper water or sea ice based on environmental DNA, leaving the composition of active microeukaryotes in the diverse AO environments largely unknown. This study provided a vertical assessment of microeukaryote communities in the AO from snow and ice to sea water at a depth of 1670 m using high-throughput sequencing of co-extracted DNA and RNA. RNA extracts depicted microeukaryote community structure and intergroup correlations more accurately and responded more sensitively to environmental conditions than those derived from DNA. Using RNA:DNA ratios as a proxy for relative activity of major taxonomic groups, the metabolic activities of major microeukaryote groups were determined along depth. Analysis of co-occurrence networks showed that parasitism between Syndiniales and dinoflagellates/ciliates in the deep ocean may be significant. This study increased our knowledge of the diversity of active microeukaryote communities and highlighted the importance of using RNA-based sequencing over DNA-based sequencing to examine the relationship between microeukaryote assemblages and the responses of microeukaryotes to environmental variables in the AO.-
dc.languageEnglish-
dc.subject.classificationAraon-
dc.titleRNA outperforms DNA-based metabarcoding in assessing the diversity and response of microeukaryotes to environmental variables in the Arctic Ocean-
dc.title.alternative북극해에서 환경변화에 따른 microeukaryoties 의 다양성-
dc.typeArticle-
dc.identifier.bibliographicCitationKong Hejun, et al. 2023. "RNA outperforms DNA-based metabarcoding in assessing the diversity and response of microeukaryotes to environmental variables in the Arctic Ocean". <em>SCIENCE OF THE TOTAL ENVIRONMENT</em>, 876(162608): 1-11.-
dc.citation.titleSCIENCE OF THE TOTAL ENVIRONMENT-
dc.citation.volume876-
dc.citation.number162608-
dc.identifier.doi10.1016/j.scitotenv.2023.162608-
dc.citation.startPage1-
dc.citation.endPage11-
dc.description.articleClassificationSCIE-
dc.description.jcrRateJCR 2021:9.319-
dc.subject.keywordArctic Ocean-
dc.subject.keywordMetabolic activity-
dc.subject.keywordMicrobial eukaryotes-
dc.subject.keywordProtists-
dc.subject.keywordco-occurrence network-
dc.identifier.localId2023-0148-
Appears in Collections  
2023-2023, Korea-Arctic Ocean Warming and Response of Ecosystem (23-23) / Yang, Eun Jin (PM23040)
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