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Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants

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Cited 33 time in scopus
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dc.contributor.authorByun, Mi Young-
dc.contributor.authorLee, Jungeun-
dc.contributor.authorCui, Li Hua-
dc.contributor.authorKang, Yoonjee-
dc.contributor.authorOh, Tae Kyung-
dc.contributor.authorPark, Hyun-
dc.contributor.authorLee, Hyoungseok-
dc.contributor.authorKim, Woo Taek-
dc.coverage.spatialAntarctic peninsula-
dc.coverage.spatialKing Sejong Station-
dc.coverage.spatialAntarctic-
dc.date.accessioned2017-08-03T13:40:14Z-
dc.date.available2017-08-03T13:40:14Z-
dc.date.issued2015-
dc.description.abstract<em>Deschampsia antarctica</em> is an Antarctic hairgrass that grows on the west coast of the Antarctic penin-sula. In this report, we have identified and characterized a transcription factor, <em>D. antarctica</em> C-repeatbinding factor 7 (DaCBF7), that is a member of the monocot group V CBF homologs. The protein con-tains a single AP2 domain, a putative nuclear localization signal, and the typical CBF signature. DaCBF7,like other monocot group V homologs, contains a distinct polypeptide stretch composed of 43 aminoacids in front of the AP2 motif. DaCBF7 was predominantly localized to nuclei and interacted withthe C-repeat/dehydration responsive element (CRT/DRE) core sequence (ACCGAC) in vitro. DaCBF7 wasinduced by abiotic stresses, including drought, cold, and salinity. To investigate its possible cellular rolein cold tolerance, a transgenic rice system was employed. DaCBF7-overexpressing transgenic rice plants(Ubi:DaCBF7) exhibited markedly increased tolerance to cold stress compared to wild-type plants withoutgrowth defects; however, overexpression of DaCBF7 exerted little effect on tolerance to drought or saltstress. Transcriptome analysis of a Ubi:DaCBF7 transgenic line revealed 13 genes that were up-regulatedin DaCBF7-overexpressing plants compared to wild-type plants in the absence of cold stress and in short-or long-term cold stress. Five of these genes, dehydrin, remorin, Os03g63870, Os11g34790, and Os10g22630,contained putative CRT/DRE or low-temperature responsive elements in their promoter regions. Theseresults suggest that overexpression of DaCBF7 directly and indirectly induces diverse genes in transgenicrice plants and confers enhanced tolerance to cold stress.-
dc.languageEnglish-
dc.subjectBiochemistry & Molecular Biology-
dc.subjectPlant Sciences-
dc.titleConstitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants-
dc.typeArticle-
dc.identifier.bibliographicCitationByun, Mi Young, et al. 2015. "Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants". <em>Plant Science,</em>, 236: 61-74.-
dc.citation.titlePlant Science,-
dc.citation.volume236-
dc.citation.page61-74.-
dc.identifier.doi10.1016/j.plantsci.2015.03.020-
dc.coverage.x62°14'29''S-
dc.coverage.y58°44'18''W-
dc.subject.keywordC-repeat/DRE binding factor-
dc.subject.keywordCold stress tolerance-
dc.subject.keywordDeschampsia antarctica-
dc.subject.keywordRNA-seq analysis-
dc.subject.keywordTransgenic rice (Oryza sativa) plant-
dc.subject.keywordAntarctic-
dc.coverage.degreeX-62.2413888888889-
dc.coverage.degreeY-58.7383333333333-
dc.identifier.scopusid2-s2.0-84928558610-
dc.identifier.wosid000356556600006-
Appears in Collections  
2014-2016, Antarctic Organisms: Cold-Adaptation Mechanism and Its Application (14-16) / Park; Hyun
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