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Starch based polyhydroxybutyrate production in engineered Escherichia coli

Cited 57 time in wos
Cited 63 time in scopus

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dc.contributor.authorBhatia, Shashi Kant-
dc.contributor.authorShim, Young-Ha-
dc.contributor.authorJeon, Jong-Min-
dc.contributor.authorBrigham, Christopher J.-
dc.contributor.authorKim, Yong-Hyun-
dc.contributor.authorKim, Hyun Joong-
dc.contributor.authorSeo, Hyung Min-
dc.contributor.authorLee, Ju Hee-
dc.contributor.authorKim, Jung-Ho-
dc.contributor.authorYi, Da-Hye-
dc.contributor.authorLee, Yoo Kyung-
dc.contributor.authorYang, Yung-Hun-
dc.date.accessioned2017-08-03T14:01:59Z-
dc.date.available2017-08-03T14:01:59Z-
dc.date.issued2014-
dc.description.abstractEvery year, the amount of chemosynthetic plastic accumulating in the environment is increasing, and significant time is required for decomposition. Bio-based, biodegradable plastic is a promising alternative, but its production is not yet a cost effective process. Decreasing the production cost of polyhydroxyalkanoate by utilizing renewable carbon sources for biosynthesis is an important aspect of commercializing this biodegradable polymer. An <em>Escherichia coli</em> strain that expresses a functional amylase and accumulate polyhydroxybutyrate (PHB), was constructed using different plasmids containing the amylase gene of <em>Panibacillus</em> sp. and PHB synthesis genes from<em> Ralstonia eutropha.</em> This engineered strain can utilize starch as the sole carbon source. The maximum PHB production (1.24 g/L) was obtained with 2 % (w/v) starch in M9 media containing 0.15 % (w/v) yeast extract and 10 mM glycine betaine. The engineered<em> E. coli </em>SKB99 strain can accumulate intracellular PHB up to 57.4 % of cell dry mass.-
dc.languageEnglish-
dc.subjectBiotechnology & Applied Microbiology-
dc.subjectEngineering-
dc.titleStarch based polyhydroxybutyrate production in engineered Escherichia coli-
dc.typeArticle-
dc.identifier.bibliographicCitationBhatia, Shashi Kant, et al. 2014. "Starch based polyhydroxybutyrate production in engineered Escherichia coli". <em>Bioprocess Biosyst Eng.</em>, 38: 1479-1484.-
dc.citation.titleBioprocess Biosyst Eng.-
dc.citation.volume38-
dc.citation.page1479-1484-
dc.identifier.doi10.1007/s00449-015-1390-y-
dc.subject.keywordAmylase-
dc.subject.keywordBiodegradable-
dc.subject.keywordPanibacillus sp.-
dc.subject.keywordPolyhydroxybutyrate-
dc.subject.keywordRalstonia eutropha-
dc.identifier.scopusid2-s2.0-84961290890-
dc.identifier.wosid000358934700006-
Appears in Collections  
2014-2016, Environmental Change Studies Based on The Arctic Dasan Station: in terms of Geology, Atmospheric Science, and Ecology (14-16) / Lee; Yookyung (PE14030; PE15030; PE16030)
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