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Involvement of a laccase-like multicopper oxidase in humic substances degradation by diverse polar soil bacteria

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dc.contributor.authorPark, Ha Ju-
dc.contributor.authorDo, Hackwon-
dc.contributor.authorLee, Jun Hyuck-
dc.contributor.authorPark, Hyun-
dc.contributor.authorKim, Dockyu-
dc.date.accessioned2017-08-03T17:57:41Z-
dc.date.available2017-08-03T17:57:41Z-
dc.date.issued2016-
dc.description.abstractSoil humic substances (HS) are widely distributed in cold environments and comprise a significant fraction of soil organic carbon. Bacterial strains (n=281) were isolated at 15°C using a minimal medium containing humic acids (HA), a principal component of HS, from various polar soil samples: 217 strains from the Antarctic and 64 strains from Arctic soil. The 73 potential HA-degrading bacteria were affiliated with phyla Proteobacteria (73.9%), Actinobacteria (20.5%), and Bacteroidetes (5.5%). Most of them degraded HA at 10°C to 25°C, but not at temperatures over 30°C, indicating their cold-adapted degradative abilities. Thirty unique laccase-like multicopper oxidase (LMCO) gene fragments were PCR-amplified from 71% of the isolated HA-degrading bacteria, all of which included the conserved copper binding regions (CBR) I and II, both essential for laccase activity. The bacterial LMCO sequences differed from known fungal laccases; for example, a cysteine residue between CBR I and CBR II of fungal laccases was not detected in the bacteria LMCOs. In addition, computer-aided molecular modeling showed these LMCOs contain a highly conserved copper-dependent active site formed by three histidine residues between CBR I and CBR II. Phylogenetic- and modeling-based methods con?rmed the wide occurrence of LMCO genes in HA-degrading polar soil bacteria and linked their putative gene functions with initial oxidative reaction for HA degradation.-
dc.languageEnglish-
dc.titleInvolvement of a laccase-like multicopper oxidase in humic substances degradation by diverse polar soil bacteria-
dc.typePoster-
dc.identifier.bibliographicCitationPark, Ha Ju, et al. 2016. Involvement of a laccase-like multicopper oxidase in humic substances degradation by diverse polar soil bacteria. ASM microbe 2016. Boston. 2016.6.16.-20.-
dc.citation.conferenceDate2016.6.16.-20-
dc.citation.conferenceNameASM microbe 2016-
dc.citation.conferencePlaceBoston-
dc.subject.keywordBiodegradation-
dc.subject.keywordBipolar-
dc.subject.keywordHumic acid-
dc.subject.keywordCold-adapted-
dc.subject.keywordLaccase-
dc.subject.keywordSoil bacteria-
Appears in Collections  
2014-2016, Antarctic Organisms: Cold-Adaptation Mechanism and Its Application (14-16) / Park; Hyun (PE14070; PE15070; PE16070)
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