Sediminicola arcticus sp. nov., a psychrophilic bacterium isolated from deep-sea sediment, and emended description of the genus Sediminicola

Abstract

A Gram-staining-negative, rod-shaped and non-motile strain, designated PAMC 27266^T , was isolated from deep-sea sediment of the Arctic Ocean. Analysis of the 16S rRNA gene sequence of strain PAMC 27266^T showed the closest affiliation with the genus <em>Sediminicola</em>. Phylogenetic analyses revealed that strain PAMC 27266^T formed a robust clade with <em>Sediminicola lutes</em> NBRC 100966T with the 16S rRNA gene sequence similarity of 98.9%. Genomic relatedness analyses based on the average nucleotide identity and the genome-to-genome distance showed that strain PAMC 27266T is clearly distinguished from <em>S. lutes</em> NBRC 100966^T. Cells of strain PAMC 27266^T grew optimally at 15℃ and pH 6.5-7.5 in the presence of 3.5% (w/v) sea salts. The major polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and two unidentified lipids. The only respiratory quinone was menaquinone-6. The major cellular fatty acids (> 10%) were C16 : 1 ω6c and/or C16 : 1ω7c and C15 : 0. The genomic DNA G+C content was 37.9 mol%. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data presented, we propose a new species for strain PAMC 27266T (= KCCM 43038^T = JCM 19894^T) with the name <em>Sediminicola arcticus </em>sp. nov.