Chemical Constituents from the Apical Bud of Gardenia sootepensis and Their Bioactivity

Abstract

Bioassay guided fractionation and isolation of the CH2Cl2 extract from the apical bud of Gardenia sootepensis (Rubiaceae) led to the isolation of five known flavonoids (1-5). The structures of the compounds were determined by 1D and 2D NMR, and MS experiments, as well as by comparison of their data with published values. Compounds 1-5 were isolated for the first time from this plant source. The isolated compounds were evaluated for their cancer chemopreventive potential based on their ability to inhibit nitric oxide (NO) production. Among the isolates, compound 4 exhibited considerable NO inhibitory activity with an IC50 value of 13.8 ？M.