Anthranilate degradation by a cold-adapted Pseudomonas sp.
DC Field | Value | Language |
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dc.contributor.author | Yoo, Miyoun | - |
dc.contributor.author | Kim, Dockyu | - |
dc.contributor.author | Hong, Soon Gyu | - |
dc.contributor.author | Kim, Eungbin | - |
dc.date.accessioned | 2018-03-20T13:41:34Z | - |
dc.date.available | 2018-03-20T13:41:34Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://repository.kopri.re.kr/handle/201206/6117 | - |
dc.description.abstract | An alpine soil bacterium Pseudomonas sp. strain PAMC 25931 was characterized as eurypsychrophilic (both psychrophilic and mesotolerant) with a broad temperature range of 530°C both for anthranilate (2-aminobenzoate) degradation and concomitant cell growth. Two degradative gene clusters (antABC and catBCA) were detected from a fosmid clone in the PAMC 25931 genomic library;each cluster was confirmed to be specifically induced by anthranilate. When expressed in Escherichia coli, the recombinant AntABC (anthranilate 1,2-dioxygenase, AntDO) converted anthranilate into catechol, exhibiting strict specificity toward anthranilate. Recombinant CatA (catechol 1,2-dioxygenase, C12O) from the organism was active over a broad temperature range (5°C-37°C). However, CatA rapidly lost the enzyme activity when incubated at above 25°C. For example, 1 hour-preincubation at 37°C resulted in 100% loss of enzyme activity, while a counterpart from mesophilic P. putida mt-2 did not show any negative effect on the initial enzyme activity. These results suggest that CatA is a new cold-adapted thermolabile enzyme, which might be a product through the adaptation process of PAMC 25931 to naturally cold environments and contribute to its ability to grow on anthranilate there. | - |
dc.language | English | - |
dc.subject | Microbiology | - |
dc.title | Anthranilate degradation by a cold-adapted Pseudomonas sp. | - |
dc.title.alternative | 적온적응성 Pseudomonas sp.에 의한 anthranilate 분해 기작 | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Yoo, Miyoun, et al. 2015. "Anthranilate degradation by a cold-adapted Pseudomonas sp.". <em>JOURNAL OF BASIC MICROBIOLOGY</em>, 55(3): 354-362. | - |
dc.citation.title | JOURNAL OF BASIC MICROBIOLOGY | - |
dc.citation.volume | 55 | - |
dc.citation.number | 3 | - |
dc.identifier.doi | 10.1002/jobm.201300079 | - |
dc.citation.startPage | 354 | - |
dc.citation.endPage | 362 | - |
dc.description.articleClassification | SCI | - |
dc.description.jcrRate | JCR 2013:70.58823529411765 | - |
dc.subject.keyword | Aromatic | - |
dc.subject.keyword | Cold-adaptation | - |
dc.subject.keyword | Degradation | - |
dc.subject.keyword | Thermolabile enzyme | - |
dc.identifier.localId | 2014-0027 | - |
dc.identifier.scopusid | 2-s2.0-84923763836 | - |
dc.identifier.wosid | 000350644000011 | - |
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