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Benzylic and aryl hydroxylations of m-xylene by o-xylene dioxygenase from Rhodococcus sp. strain DK17

Cited 10 time in wos
Cited 11 time in scopus
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dc.contributor.authorKim, Dockyu-
dc.contributor.authorJung Nam Choi-
dc.contributor.authorZylstra, Gerben J.-
dc.contributor.authorKim, Eungbin-
dc.contributor.authorKang, Beom Sik-
dc.contributor.authorChoong Hwan Lee-
dc.contributor.authorYoo, Miyoun-
dc.contributor.authorKi Young Choi-
dc.date.accessioned2018-03-20T13:42:09Z-
dc.date.available2018-03-20T13:42:09Z-
dc.date.issued2010-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6135-
dc.description.abstractEscherichia coli cells expressing Rhodococcus DK17 o-xylene dioxygenase genes were used for bioconversion of m-xylene. Gas chromatography-mass spectrometry analysis of the oxidation products detected 3 methylbenzylalcohol and 2,4-dimethylphenol in the ratio 9:1. Molecular modeling suggests that o-xylene dioxygenase can hold xylene isomers at a kink region between α6 and α7 helices of the active site and α9 helix covers the substrates. m-Xylene is unlikely to locate at the active site with a methyl group facing the kink region because this configuration would not fit within the substrate-binding pocket. The m-xylene molecule can flip horizontally to expose the meta-position methyl group to the catalytic motif. In this configuration, 3 methylbenzylalcohol could be formed, presumably due to the meta effect. Alternatively, the m-xylene molecule can rotate counterclockwise, allowing the catalytic motif to hydroxylate at C-4 yielding 2,4-dimethylphenol. Site-directed mutagenesis combined with structural and functional analyses suggests that the alanine-218 and the aspartic acid-262 in the α7 and the α9 helices play an important role in positioning m-xylene, respectively-
dc.languageEnglish-
dc.publisherSpringer Berlin / Heidelberg-
dc.subjectBiotechnology & Applied Microbiology-
dc.titleBenzylic and aryl hydroxylations of m-xylene by o-xylene dioxygenase from Rhodococcus sp. strain DK17-
dc.title.alternativeRhodococcus sp. strain DK17이 생산하는 o-xylene dioxygenase에 의한 m-xylene의 benzylic- 및 aryl-hydroxylation 반응-
dc.typeArticle-
dc.identifier.bibliographicCitationKim, Dockyu, et al. 2010. "Benzylic and aryl hydroxylations of m-xylene by o-xylene dioxygenase from Rhodococcus sp. strain DK17". <em>APPLIED MICROBIOLOGY AND BIOTECHNOLOGY</em>, 86(6): 1841-1847.-
dc.citation.titleAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY-
dc.citation.volume86-
dc.citation.number6-
dc.identifier.doi10.1007/s00253-009-2418-5-
dc.citation.startPage1841-
dc.citation.endPage1847-
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2008:33.33333333333333-
dc.subject.keywordBenzylic hydroxylation-
dc.subject.keywordRhodococcus-
dc.subject.keywordmeta Effect-
dc.subject.keywordo-Xylene dioxygenase-
dc.identifier.localId2010-0033-
dc.identifier.scopusid2-s2.0-77952882068-
dc.identifier.wosid000276960700021-
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2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han
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