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Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial

Cited 16 time in wos
Cited 18 time in scopus
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dc.contributor.authorLee, Yung Mi-
dc.contributor.authorLee, Hong Kum-
dc.contributor.authorKim, Eun Hye-
dc.contributor.authorCho, Kyeung Hee-
dc.contributor.authorHong, Soon Gyu-
dc.contributor.authorCho, Jang-Cheon-
dc.contributor.authorYim, Joung Han-
dc.coverage.spatialArctic-
dc.date.accessioned2018-03-20T13:44:06Z-
dc.date.available2018-03-20T13:44:06Z-
dc.date.issued2010-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6183-
dc.description.abstractA new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from Svalbard, Norway. The method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. ZoBell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. One to three types of colonies were observed from each enrichment culture, indicating that specific bacterial species were enriched during the experimental conditions. Among 89 bacterial isolates, protease activity was observed from 48 isolates in the screening media containing skim milk. Good growth was observed at 4C and 10C while none of the isolates could grow at 37C. At low temperatures, enzyme activity was equal to or higher than activity at higher temperatures. Bacterial isolates were included in the genera Pseudoalteromonas (33 isolates), Arthrobacter (24 isolates), Pseudomonas (16 isolates), Psychrobacter (6 isolates), Sphingobacterium (6 isolates), Flavobacterium (2 isolates), Sporosarcina (1 isolate), and Stenotrophomonas (1 isolate). Protease activity was observed from Pseudoalteromonas (33 isolates), Pseudomonas (10 isolates), Arthrobacter (4 isolates), and Flavobacterium (1 isolate).-
dc.languageEnglish-
dc.publisherSpringer-
dc.subjectMicrobiology-
dc.titleDiversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial-
dc.title.alternative북극환경에서 농화배양에 의해 분리된 저온활성 단백질분해효소 생산균주의 다양성-
dc.typeArticle-
dc.identifier.bibliographicCitationLee, Yung Mi, et al. 2010. "Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial Diversity of Cold-Active Protease-Producing Bacteria from Arctic Terrestrial". <em>JOURNAL OF MICROBIOLOGY</em>, 48(4): 426-432.-
dc.citation.titleJOURNAL OF MICROBIOLOGY-
dc.citation.volume48-
dc.citation.number4-
dc.identifier.doi10.1007/s12275-010-0015-z-
dc.citation.startPage426-
dc.citation.endPage432-
dc.description.articleClassificationSCIE-
dc.description.jcrRateJCR 2008:78.02197802197803-
dc.subject.keywordArctic-
dc.subject.keywordenrichment-
dc.subject.keywordprotease-
dc.identifier.localId2010-0085-
dc.identifier.scopusid2-s2.0-77956131467-
dc.identifier.wosid000281516800003-
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han
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