A new method for microbial cultivation and its application to bacterial community analysis in Buus Nuur, Mongolia

Abstract

We performed a comparative analysis ofbacterial di 、ers ity in a saline soda lake lIsing a novel clIltivation method termed the f？ lter plate microbial σap method (FPMT), and conventional techniqlles together with DGGE (denaturing gradient gel eleclrophoresis). The new method allowed for cllltivat10n of representatives of se、en bacterial taxonomic grollps, incJuding seven noveJ species. This compares favorably to the reslIJts from cllltivation of representatives of four taxonomic groups and onJy one novel species using standard approaches. Neither culture collection matched the community composition revealed by DGGE but the FPMT method produced a more representative collection of strains. We conclude that FPMT is a powerω 1 device for improving bacterial c비turability and narrowing the gap between microbial diversity in nature and cllltivable microorganisms.ng gradient gel eleclrophoresis). The new method allowed for cllltivat10n of representatives of se、en bacterial taxonomic grollps, incJuding seven noveJ species. This compares favorably to the reslIJts from cllltivation of representatives of four taxonomic groups and onJy one novel species using standard approaches. Neither culture collection matched the community composition revealed by DGGE but the FPMT method produced a more representative collection of strains. We conclude that FPMT is a powerω 1 device for improving bacterial c비turability and narrowing the gap between microbial diversity in nature and cllltivable microorganisms.