Aromatic hydroxylation of indan by the o-xylene-degrading Rhodococcus sp. strain DK17

Abstract

The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o xylene pathway. Metabolite and RT-PCR analyses indicate that o xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.