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Differential degradation of bicyclics with aromatic and alicyclic rings by Rhodococcus sp. strain DK17

Cited 11 time in wos
Cited 13 time in scopus
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dc.contributor.authorKang, Beom Sik-
dc.contributor.authorHong, Soon Gyu-
dc.contributor.authorYoo, Miyoun-
dc.contributor.authorKim, Dockyu-
dc.contributor.authorKim, Eungbin-
dc.contributor.authorZylstra, Gerben J.-
dc.contributor.authorKi Young Choi-
dc.contributor.authorKim, Tai Kyoung-
dc.date.accessioned2018-03-20T13:54:43Z-
dc.date.available2018-03-20T13:54:43Z-
dc.date.issued2011-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6425-
dc.description.abstractThe metabolically versatile Rhodococcus sp. strain DK17 is able to grow on tetralin and indan but cannot use their respective desaturated counterparts, 1,2-dihydronaphthalene and indene, as sole carbon and energy sources. Metabolite analyses by gas chromatography-mass spectrometry and nuclear magnetic resonance spectrometry clearly show that (1) the meta-cleavage dioxygenase mutant strain DK180 accumulates 5,6,7,8-tetrahydro-1,2-naphthalene diol, 1,2-indene diol, and 3,4-dihydro-naphthalene-1,2-diol from tetralin, indene, and 1,2-dihydronaphthalene, respectively and (2) when expressed in E. coli, the DK17 o-xylene dioxygenase transforms tetralin, indene, and 1,2-dihydronaphthalene into tetralin cis-dihydrodiol, indan-1,2-diol, and cis-1,2-dihydroxy-1,2,3,4-tetrahydronaphthalene, respectively. Tetralin, which is activated by aromatic hydroxylation, is degraded successfully via the ring-cleavage pathway to support growth of DK17. Indene and 1,2-dihydronaphthalene do not serve as growth substrates because DK17 hydroxylates them on the alicyclic ring and further metabolism results in a dead-end metabolite. This study reveals that aromatic hydroxylation is a prerequisite for proper degradation of bicyclics with aromatic and alicyclic rings by DK17, and confirms the unique ability of the DK17 o-xylene dioxygenase to perform distinct regioselective hydroxylations.-
dc.languageEnglish-
dc.publisherAmerican Society for Microbiology-
dc.subjectBiotechnology & Applied Microbiology-
dc.subjectMicrobiology-
dc.titleDifferential degradation of bicyclics with aromatic and alicyclic rings by Rhodococcus sp. strain DK17-
dc.title.alternativeRhodococcus sp. strain DK17에 의한 bicyclic rings의 분해에 필수적 hydroxylation 반응-
dc.typeArticle-
dc.identifier.bibliographicCitationKang, Beom Sik, et al. 2011. "Differential degradation of bicyclics with aromatic and alicyclic rings by Rhodococcus sp. strain DK17". <em>Applied and Environmental Microbiology</em>, 77(23): 8280-8287.-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.volume77-
dc.citation.number23-
dc.identifier.doi10.1128/AEM.06359-11-
dc.citation.startPage8280-
dc.citation.endPage8287-
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2009:19.078947368421055-
dc.subject.keyword2-dihydronaphthalene-
dc.subject.keywordRhodococcus-
dc.subject.keyworddegradative pathway-
dc.subject.keywordindene-
dc.subject.keywordtetralin-
dc.identifier.localId2011-0261-
dc.identifier.scopusid2-s2.0-83255188017-
dc.identifier.wosid000297164100011-
Appears in Collections  
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2011-2013, Utilization of novel metabolites from polar organisms (11-13) / Yim, Joung Han
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