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Pilot-scale production of the antifreeze protein from Antarctic bacterium Flavobacterium frigoris PS1 by recombinant Escherichia coli with a cold shock induction system

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dc.contributor.authorKim, Eun Jae-
dc.contributor.authorHan, Se Jong-
dc.contributor.authorLee, Sung Gu-
dc.contributor.authorLee, Jun Hyuck-
dc.date.accessioned2018-03-20T14:02:30Z-
dc.date.available2018-03-20T14:02:30Z-
dc.date.issued2015-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6580-
dc.description.abstractAntifreeze proteins (AFP) inhibit growth and recrystallization of ice, and permit organisms to survive in cold environments. The AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris ice-binding protein), was produced in E. coli using a cold shock induction system. The culture temperature was shifted from 37°C to 15°C and a 20 L culture scale was used. The final weights of dried cell and FfIBP were estimated to be 126 g and 8.4 g, respectively. The thermal hysteresis (TH) activity (1.53°C) of the produced FfIBP was 3.6-fold higher than that of the LeIBP (Leucosporidium ice-binding protein) produced in Picha. The current study demonstrates that large-scale production of FfIBP was successful and the result could be extended to further application studies using recombinant AFPs.-
dc.languageKorean-
dc.subjectEngineering-
dc.titlePilot-scale production of the antifreeze protein from Antarctic bacterium Flavobacterium frigoris PS1 by recombinant Escherichia coli with a cold shock induction system-
dc.title.alternative저온 유도 시스템을 가진 재조합 대장균을 이용한 남극 세균 Flavobacterium frigoris PS1 유래 결빙방지단백질의 Pilot-scale 생산-
dc.typeArticle-
dc.identifier.bibliographicCitationKim, Eun Jae, et al. 2015. "Pilot-scale production of the antifreeze protein from Antarctic bacterium Flavobacterium frigoris PS1 by recombinant Escherichia coli with a cold shock induction system". <em>KSBB Journal</em>, 30(6): 345-349.-
dc.citation.titleKSBB Journal-
dc.citation.volume30-
dc.citation.number6-
dc.identifier.doi10.7841/ksbbj.2015.30.6.345-
dc.citation.startPage345-
dc.citation.endPage349-
dc.description.articleClassificationKCI등재-
dc.description.jcrRateJCR 2013:0-
dc.subject.keywordAntifreeze protein-
dc.subject.keywordCold shock induction-
dc.subject.keywordFlavobacterium frigoris-
dc.subject.keywordPilot scale fermentation-
dc.identifier.localId2015-0247-
Appears in Collections  
2013-2015, Production of bioenergy using cold resistance polar organisms (13-15) / Han, Se Jong (PE13150; PE14140; PE15140)
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