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Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities

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dc.contributor.authorPark, Ha Ju-
dc.contributor.authorKim, Dockyu-
dc.contributor.authorLee, Yung Mi-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorLee, Hong Kum-
dc.contributor.authorHong, Soon Gyu-
dc.coverage.spatialAntarctic-
dc.coverage.spatialArctic-
dc.date.accessioned2018-03-20T14:06:20Z-
dc.date.available2018-03-20T14:06:20Z-
dc.date.issued2010-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/6666-
dc.description.abstractThe Korea Polar Research Institute (KOPRI) has assembled a culture collection of cold-adapted bacterial strains from both the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected in advance according to their relative activities and were subsequently re-examined for their extracellular protease activity on 0.1×ZoBell plates supplemented with 1% skim milk at various temperatures. This rapid and direct screening method permitted the selection of a small group of 15 cold-adapted bacterial strains,belonging to either the genus Pseudoalteromonas (13 strains) or Flavobacterium (2 strains), that showed proteolytic activities at temperatures ranging between 5-15?°C. The cold-active proteases from these strains were classified into four categories (serine protease, aspartic protease, cysteine protease, and metalloprotease) according to the extent of enzymatic inhibition by a class-specific protease inhibitor.Since highly active and/or cold-adapted proteases have the potential for industrial or commercial enzyme development, the protease-producing bacteria selected in this work will be studied as a valuable natural source of new proteases. Our results also highlight the relevance of the Antarctic for the isolation of protease-producing bacteria active at low temperatures.-
dc.languageKorean-
dc.subjectLife Sciences & Biomedicine - Other Topics-
dc.titleScreening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities-
dc.title.alternative남북극 유래 저온성 박테리아 culture collection에서 저온활성 프로테아제 생산균주의 스크리닝과 효소 특성-
dc.typeArticle-
dc.identifier.bibliographicCitationPark, Ha Ju, et al. 2010. "Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities". <em>The Microbiological Society Of Korea</em>, 46(1): 73-79.-
dc.citation.titleThe Microbiological Society Of Korea-
dc.citation.volume46-
dc.citation.number1-
dc.citation.startPage73-
dc.citation.endPage79-
dc.description.articleClassificationKCI등재-
dc.description.jcrRateJCR 2008:0-
dc.subject.keywordAntarctic-
dc.subject.keywordArctic-
dc.subject.keywordcold-active-
dc.subject.keywordculture collection-
dc.subject.keywordprotease-
dc.identifier.localId2010-0026-
dc.identifier.scopusid2-s2.0-77955796743-
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han
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