Cultivation and Lipid Formation of a Green Microalgae, Chlamydomonas sp. ArM0029A, Isolated from Arctic Sea Ice

Abstract

Chlamydomonas sp. ArM0029A originated from Arctic sea ice was cultivated for the production of biofuel. The pure culture was identified by sequence analysis (18s rDNA, rbcL gene) and the high lipid-producing microalgae Chlamydomonas sp. ArM0029A was examined by using nile red and fluorescence microscopy. Chlamydomonas sp. ArM0029A was grown at 4℃, 8℃, 12℃and 20℃, the doubling times were 72h, 34h,18h, and 13h, at 4℃, 8℃, 12℃and 20℃, respectively. The maximal cell densities were 4×107, 2.6×107, 2×107, and 1.5×107 cells/mL at 4℃, 8℃, 12℃and 20℃, respectively. Cells were visualized by nile red staining method and the lipid body dyed yellow-gold at 488nm fluorescence. The total lipid was analyzed by LC-MS and it was found that Chlamydomonas sp. ArM0029A contained more phospholipid, triglycerides (TG) and diacylglycerols (DG) than the control strain, (Chlamydomonas reinhardtii CC-125), in positive mode. Taken together, these results suggest that ArM0029A may be a source of biodiesel and functional lipids. [This work was supported by Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (PN13120).]