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Isolation and characterisation of metallothionein from Antarctic clam Laternula elliptica

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dc.contributor.authorPark, Hyun-
dc.contributor.author표세홍-
dc.contributor.authorAhn, In-Young-
dc.contributor.author이혜은-
dc.coverage.spatialAntarctic-
dc.date.issued2006-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7661-
dc.description.abstractAntarctic clam, Laternula elliptica, adapted for survival in high concentrations of heavy metal induce metallothionein in the presence of Cadmium. In this study, we isolated and characterized a cadmium-binding protein, metallothionein, from metal-exposed clams, L. elliptica. Purification by gel-permeation and ion-exchange chromatography showed that the metal is bound principally to newly formed proteins. The molecular weight of the purified metallothionein was determined as 7.27 kDa by MALDI-TOF MS analyses. The absorption spectra profiles indicate Cd-metallothioneins complexes, and there is a 4 nm red shift of the unresolved lowest energy absorption band when 5 equiv of Cd(II) is incorporated.-
dc.languageEnglish-
dc.titleIsolation and characterisation of metallothionein from Antarctic clam Laternula elliptica-
dc.title.alternative남극큰띠조개로부터 metallothionein 분리정제-
dc.typeProceeding-
dc.identifier.bibliographicCitationPark, Hyun, et al. 2006. Isolation and characterisation of metallothionein from Antarctic clam Laternula elliptica. 2006.05.09~.-
dc.citation.volume00-
dc.citation.number00-
dc.citation.conferenceDate2006.05.09~-
dc.description.articleClassificationPro(초록)국외-
dc.subject.keywordAntarctic-
dc.subject.keywordLaternula elliptica-
dc.subject.keywordmetallothionein-
dc.identifier.localId2006-0158-
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2005-2007, Monitoring on Environmental Changes at the Korean Arctic and Antarctic Stations (05-07) / Ahn, In-Young
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