KOPRI Repository

Semipurification of Ice-crystal Controlling Protein from the Arctic Bacterium Aquaspirillum sp. KOPRI-AB30

Cited 0 time in wos
Cited 0 time in scopus

Full metadata record

DC Field Value Language
dc.contributor.author박승일-
dc.contributor.authorKim, Hak Jun-
dc.contributor.authorSeo, Ki-Won-
dc.contributor.authorKang, Sung-Ho-
dc.date.issued2007-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7710-
dc.description.abstractAntifreeze proteins(AFPs) bind to the surface of ice crystals and control ice crystal structure. We islated some bacteria capable of producing antifreeze proteins as part of cold adaptation mechanism from the Arctic. Among these, the strain KOPRI-AB30 showed high antifreeze activity and was identified as Aquaspirillum sp. The psychrophilic bacterium Aquaspirillum sp.KoPRI-AB30 produced antifreeze protein extracellularly. The optimal temperature and pH for the production of AFP were 5℃ and 7.0, respectively. The AFP was partially purified from the culture supernatant with tangential flow filtration and subsequent chromatography. Molecular mass of the partially-purifed AFP was approximately 20kDa. The ice-crystal controlling activity of the AFP was observed using nanooliter osmometer. The thermal hysteresis of the protein was determined to be about 0.7℃. In addition, the AFP is relatively heat and pH stable, but not calcium dependant. The possible use of the protein as a cryoprotectant was also tested.-
dc.languageEnglish-
dc.titleSemipurification of Ice-crystal Controlling Protein from the Arctic Bacterium Aquaspirillum sp. KOPRI-AB30-
dc.typeProceeding-
dc.identifier.bibliographicCitation박승일, et al. 2007. Semipurification of Ice-crystal Controlling Protein from the Arctic Bacterium Aquaspirillum sp. KOPRI-AB30. Cryobiology. Cryobiology. 2007.07.30~.-
dc.citation.volume0-
dc.citation.number0-
dc.citation.conferenceDate2007.07.30~-
dc.citation.conferenceNameCryobiology-
dc.citation.conferencePlaceCryobiology-
dc.description.articleClassificationPro(초록)국외-
dc.subject.keywordantifreeze proteins-
dc.subject.keywordice recrystallization-
dc.subject.keywordice-structuring proteins-
dc.subject.keywordthermal hysteresis-
dc.identifier.localId2007-0075-
Appears in Collections  
2006-2010, Research on culturable polar organisms and their application (06-10) / Kang, Sung-Ho; Choi, Han-Gu (PE06060, PE07060, PE08060, PE09060, PE10060)
Files in This Item
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse