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Functional Expression and Characterization of Recombinant Chitinase from Antarctic Psychrophilic Bacterium Sanguibacter antarcticus KCTC 13143

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dc.contributor.author이창은-
dc.contributor.authorKim, Il-Chan-
dc.contributor.author김주영-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorKim, Dockyu-
dc.contributor.authorPark, Ha Ju-
dc.date.issued2008-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7873-
dc.description.abstractA psychrophilic Sanguibacter antarcticus KCTC 13143 has been previously isolated from sea sand on King Sejong Station in Antarctica. An cold-active endochitinase gene, designated chi21702, from KCTC 13143 was PCR-amplified, cloned into pEXP5-CT/TOPO vector, and overexpressed in Eschrichia coli BL21(DE3) without its signal-peptide and with an C-terminal His-tag. The recombinant chitinase (R-Chi21702) was approximately 56 kDa in size, most of which was converted to inclusion body having no chitinae activity against oligomers of N-acetylglucosamine. In next step, refolding experiment for the inert chitinase was carried out using optimized refolding conditions. The refolded R-Chi21702 was then purified using a Ni-NTA column, which restored its chitinase specific activity (5.0 U/mg). The optimal reaction temperature and pH of R-chitinase were determined to be 40 ℃ and pH 8.0, respectively.-
dc.languageEnglish-
dc.titleFunctional Expression and Characterization of Recombinant Chitinase from Antarctic Psychrophilic Bacterium Sanguibacter antarcticus KCTC 13143-
dc.title.alternative남극 호냉성 세균 Sanguibacter antarcticus KCTC 13143의 재조합 키틴분해효소의 발현 및 특성연구-
dc.typeProceeding-
dc.identifier.bibliographicCitation이창은, et al. 2008. Functional Expression and Characterization of Recombinant Chitinase from Antarctic Psychrophilic Bacterium Sanguibacter antarcticus KCTC 13143. 극지연구소. 극지연구소. 2008.09.25~.-
dc.citation.volume1-
dc.citation.number1-
dc.citation.conferenceDate2008.09.25~-
dc.citation.conferenceName극지연구소-
dc.citation.conferencePlace극지연구소-
dc.description.articleClassificationPro(초록)국외-
dc.subject.keywordCold-active-
dc.subject.keywordEndochitinase-
dc.subject.keywordPsychrophilic-
dc.subject.keywordSanguibacter-
dc.identifier.localId2008-0236-
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han (PE06050, PE07050, PE08050, PE09050, PE10050)
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