KOPRI Repository

Characterization and chemical modification of a GlcNAc/GalNAc specific lectin, Bryohealin, from the marine green alga Bryopsis plumosa

Cited 0 time in wos
Cited 0 time in scopus

Full metadata record

DC Field Value Language
dc.contributor.authorJung, Min Gui-
dc.contributor.authorChoi, Han-Gu-
dc.contributor.authorKang, Sung-Ho-
dc.contributor.authorKim, Gwang Hoon-
dc.contributor.author이기평-
dc.date.issued2009-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7898-
dc.description.abstractBryohealin is lectin isolated from the marine green alga Bryopsis plumosa. It was reported to be involved in the wound-healing process of B. plumosa. The lectin molecule is composed of two identical subunits of 27 kDa each, cross-linked by disulfide bond and showed binding specificity to N-acetyl-D-glucosamine and N-acetyl-D-galactosamine. We demonstrated characterization of a carbohydrate binding site of Bryohealin by chromatography and chemical modification methods. Bryohealin had identical two or more binding sites and shared same binding site for two different carbohydrates (GalNAc and GlcNAc). During chemical modification studies, hemagglutinating activities of Bryohealin were not affected by modification of histidine, tryptophan, asparticacid, and glutamicacid. When 50% of arginine residues were modified with 1, 2-cyclohexanedione, activity of Bryohealin rapidly decreased. Protection was observed when the arginine modification was performed in the presence of inhibitory sugar (0.15 M GalNAc). The results suggest that the modified arginine is included in the carbohydrate binding site of Bryohealin. Tryptic digested peptides containing modified arginine residues were separated by HPLC.-
dc.languageEnglish-
dc.titleCharacterization and chemical modification of a GlcNAc/GalNAc specific lectin, Bryohealin, from the marine green alga Bryopsis plumosa-
dc.title.alternative해양 녹조식물 Bryopsis plumosa로 부터 정제한 lectin, Bryohealin의 당 결합 자리 특성 연구-
dc.typeProceeding-
dc.identifier.bibliographicCitationJung, Min Gui, et al. 2009. Characterization and chemical modification of a GlcNAc/GalNAc specific lectin, Bryohealin, from the marine green alga Bryopsis plumosa. 한국조류학회. 한국조류학회. 2009.06.05~.-
dc.citation.volume23-
dc.citation.number2-
dc.citation.conferenceDate2009.06.05~-
dc.citation.conferenceName한국조류학회-
dc.citation.conferencePlace한국조류학회-
dc.description.articleClassificationPro(초록)국내-
dc.subject.keywordBryohealin-
dc.subject.keywordBryopsis plumosa-
dc.subject.keywordN-acetyl-D-galactosamine-
dc.subject.keywordN-acetyl-D-glucosamine-
dc.subject.keywordlectin-
dc.identifier.localId2009-0045-
Appears in Collections  
2006-2010, Research on culturable polar organisms and their application (06-10) / Kang, Sung-Ho; Choi, Han-Gu (PE06060, PE07060, PE08060, PE09060, PE10060)
Files in This Item
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse