Production of psychrophilic chitinase from Antarctic microorganism by recombinant Escherichia coli

Abstract

In order to enhance the production of psychrophilic chitinase, recombinant Escherichia coli Top 10 harboring chitinase gene from Antarctic microorganism KOPRI 22718 was cultivated using flask and jar fermentor. Expression of target protein was performed by L-arabinose addition and confirmed by SDS-PAGE and activity test with p-nitrophenyl-N-acetyl-&#61538

-D-glucosaminide as a substrate. To increase the cell density and production of enzyme, modified R-medium was tested as a fermentation medium and effect of L-arabinose concentration was also observed. About 28 times higher enzyme production was obtained by using modified R-medium with lactose and 2.5g/L of L-arabinose in fermentor culture than that by using LB medium in flask culture.