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The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33

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dc.contributor.authorHan, Se Jong-
dc.contributor.authorPark, Heeyong-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorKoh, Hye Yeon-
dc.contributor.authorLee, Sung Gu-
dc.description.abstractTo confirm the optimal cultivation conditions of Pichia pastoris X-33 containing the psychrophilic chitinase gene cloned from Antarctic microorganism, Sanguibacter antarcticus KOPRI 21702, the effect of temperature and pH on chitinase expression was investigated in a jar fermentor. Expression of target protein was performed by methanol addition and confirmed by an activity assay with p-nitrophenyl-N-acetyl-&#61538-
dc.description.abstract-D-glucosaminide as a substrate. Cells were grown at various temperatures ranging from 20 to 30 &#61616-
dc.description.abstractC and pHs from 5.5 to 6.5. The chitinase production was divided into two phases: batch and induction fed-batch. Ultimately, the optimal temperature and pH for the production of chitinase were found to be 20 &#61616-
dc.description.abstractC, pH 6.0, respectively.-
dc.titleThe effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33-
dc.title.alternative재조합 효모에 의한 저온성 chitinase의 발현에 미치는 온도 및 pH의 영향-
dc.identifier.bibliographicCitationHan, Se Jong, et al. 2009. The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33. (사) 한국생물공학회. (사) 한국생물공학회. 2009.11.02~.-
dc.citation.conferenceName(사) 한국생물공학회-
dc.citation.conferencePlace(사) 한국생물공학회-
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han
2009-2010, 극지 미생물 유래 재조합 단백질 생산 시스템 개발 (09-10) / Han, Se Jong
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