KOPRI Repository

The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33

Cited 0 time in wos
Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHan, Se Jong-
dc.contributor.authorPark, Heeyong-
dc.contributor.authorYim, Joung Han-
dc.contributor.authorKoh, Hye Yeon-
dc.contributor.authorLee, Sung Gu-
dc.coverage.spatialAntarctic-
dc.date.issued2009-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/7962-
dc.description.abstractTo confirm the optimal cultivation conditions of Pichia pastoris X-33 containing the psychrophilic chitinase gene cloned from Antarctic microorganism, Sanguibacter antarcticus KOPRI 21702, the effect of temperature and pH on chitinase expression was investigated in a jar fermentor. Expression of target protein was performed by methanol addition and confirmed by an activity assay with p-nitrophenyl-N-acetyl-&#61538-
dc.description.abstract-D-glucosaminide as a substrate. Cells were grown at various temperatures ranging from 20 to 30 &#61616-
dc.description.abstractC and pHs from 5.5 to 6.5. The chitinase production was divided into two phases: batch and induction fed-batch. Ultimately, the optimal temperature and pH for the production of chitinase were found to be 20 &#61616-
dc.description.abstractC, pH 6.0, respectively.-
dc.languageEnglish-
dc.titleThe effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33-
dc.title.alternative재조합 효모에 의한 저온성 chitinase의 발현에 미치는 온도 및 pH의 영향-
dc.typeProceeding-
dc.identifier.bibliographicCitationHan, Se Jong, et al. 2009. The effect of temperature and pH on psychrophilic chitinase expression in Pichia pastoris X-33. (사) 한국생물공학회. (사) 한국생물공학회. 2009.11.02~.-
dc.citation.volume25-
dc.citation.conferenceDate2009.11.02~-
dc.citation.conferenceName(사) 한국생물공학회-
dc.citation.conferencePlace(사) 한국생물공학회-
dc.description.articleClassificationPro(초록)국내-
dc.subject.keywordAntarctic-
dc.subject.keywordPichia-
dc.subject.keywordchitinase-
dc.subject.keywordpsychrophilic-
dc.identifier.localId2009-0169-
Appears in Collections  
2006-2010, Procurement and utilization of polar genetic resources (06-10) / Lee, Hong Kum; Yim, Joung Han
2009-2010, 극지 미생물 유래 재조합 단백질 생산 시스템 개발 (09-10) / Han, Se Jong
Files in This Item
There are no files associated with this item.
General Conditions
      ROMEO Green
    Can archive pre-print and post-print or publisher's version/PDF
      ROMEO Blue
    Can archive post-print (ie final draft post-refereeing) or publisher's version/PDF
      ROMEO Yellow
    Can archive pre-print (ie pre-refereeing)
      ROMEO White
    Archiving not formally supported

    Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

    Browse