The complete mitochondrial genome sequences of Trinorchestia longiramus (Crustacea:Amphipoda:Talitridae).

Abstract

The talitrid amphipods are common in sand beaches, estuarine marshes, shores of lakes and rivers around the world. They are detritivores and preys for birds and other animals, and play an important role in the food chain of ecosystem. Despite of their significance and vast diversity, no complete mitochondrial genome data have been available so far. Mitochondrial genomes contain the most informative sequences and gene arrangement for deeper phylogenetic analyses and they reflect evolutionary relationships and biogeography in the metazonas. In the present study, we describe the mitochondrial genome (mitogenome) sequences of talitrid Trinorchestia longiramus. T. longiramus was collected from the east coastal area in Korea. To analyze the mitogenome of the talitrid, we obtained the sequences of CO1, 12S, 16S, CO3 and Cytb in each talitrid using universal primers published or newly designed in our group and then, amplified the complete mitogenome of using long-PCR and genome walking techniques. As it has been reported that some species in extreme environment show unusual mt genome composition and structure, we attempted to compare mitochondrial genome feature of the species inhabiting in Korea. Also, we attempted to solve the ordinal relationships of Amphipoda in Class Malacostraca of Subphylum Crustacea by phylogenetic analysis using sequence data from mitochondrial protein-coding genes. Our respite of their significance and vast diversity, no complete mitochondrial genome data have been available so far. Mitochondrial genomes contain the most informative sequences and gene arrangement for deeper phylogenetic analyses and they reflect evolutionary relationships and biogeography in the metazonas. In the present study, we describe the mitochondrial genome (mitogenome) sequences of talitrid Trinorchestia longiramus. T. longiramus was collected from the east coastal area in Korea. To analyze the mitogenome of the talitrid, we obtained