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Properties of phase transition of ice binding protein from Arctic yeast (LeIBP) utilizing differential scanning calorimetry (DSC) and Raman spectroscopy

Cited 5 time in wos
Cited 5 time in scopus

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dc.contributor.authorLee, Sanghwa-
dc.contributor.authorHong, Jong Wook-
dc.contributor.authorKim, Han-Woo-
dc.contributor.authorLee, Jun Hyuck-
dc.date.accessioned2019-01-18T04:34:21Z-
dc.date.available2019-01-18T04:34:21Z-
dc.date.issued2018-12-
dc.identifier.issn0011-2240-
dc.identifier.urihttps://repository.kopri.re.kr/handle/201206/9526-
dc.description.abstractIce binding proteins (IBPs) have been attracting significant interest on account of their characteristic of inhibiting ice growth and recrystallization. Owing to their unique characteristics, IBPs have been studied for applications in food, pharmaceuticals, and medicine, as well as from a general scientific point of view. In this study, we have used differential scanning calorimetry (DSC) and Raman spectroscopy as tools to understand the ice binding activity of the Arctic-yeast-originating extracellular ice binding glycoprotein (LeIBP) isolated from Leucosporidium sp. AY30. From the DSC results, an increase in the specific heat capacity was confirmed for 1 mg/mL LeIBP, which suggested that additional heat flow was required for the change in temperature. In addition, the temperature corresponding to the phase change of the solution was measured, and Raman spectroscopy was carried out on the frozen and molten phases, respectively. From the results of Raman analysis, we confirmed that the helical vibrations related to the ice binding sites on LeIBP were dramatically suppressed when the LeIBP solution was frozen. Furthermore, principal component analysis (PCA) of the Raman spectra yielded the contrast factor between the freezing and melting states. Both DSC and Raman spectroscopy are widely used to study the ice binding activity and the structural changes associated with molecular vibrations in cryobiology.en_US
dc.languageEnglish-
dc.language.isoenen_US
dc.subjectLife Sciences & Biomedicine - Other Topics; Physiologyen_US
dc.subject.classificationDasan Stationen_US
dc.titleProperties of phase transition of ice binding protein from Arctic yeast (LeIBP) utilizing differential scanning calorimetry (DSC) and Raman spectroscopyen_US
dc.title.alternativeDSC와 Raman spectroscopy 방법을 이용한 북극 효모유래 얼음결합단백질의 얼음결합기작 연구en_US
dc.typeArticleen_US
dc.identifier.bibliographicCitationLee, Sanghwa, et al. 2018. "Properties of phase transition of ice binding protein from Arctic yeast (LeIBP) utilizing differential scanning calorimetry (DSC) and Raman spectroscopy". <em>CRYOBIOLOGY</em>, 85(1): 33-38.-
dc.citation.titleCRYOBIOLOGYen_US
dc.citation.volume85en_US
dc.citation.number1en_US
dc.identifier.doi10.1016/j.cryobiol.2018.10.005-
dc.citation.startPage33en_US
dc.citation.endPage38en_US
dc.description.articleClassificationSCI-
dc.description.jcrRateJCR 2016:35.2941176470588en_US
dc.subject.keywordAntifreeze proteinen_US
dc.subject.keywordLeIBPen_US
dc.subject.keywordRaman spectroscopyen_US
dc.subject.keyworddifferential scanning calorimetry (DSC)en_US
dc.subject.keywordprincipal component analysis (PCA)en_US
dc.identifier.localId2018-0208-
dc.identifier.scopusid2-s2.0-85054715280-
dc.identifier.wosid000454851300006-
Appears in Collections  
2018-2018, Development of potential candidates as antibiotics based on polar genetic resources (18-18) / Lee, Jun Hyuck (PE18210)
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