PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis
Cited 2 time in
Cited 3 time in
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Title
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PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis
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Other Titles
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북극 미생물 Paenibacillus sp R4 유래의 저온성 에스테라제 PsEst3: 결정화와 X-ray 결정 분석
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Authors
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Kim, Hyun
Park, Ae Kyung
Lee, Jun Hyuck
Shin, Seung Chul
Park, Hyun
Kim, Han-Woo
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Subject
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Biochemistry & Molecular Biology; Biophysics; Crystallography
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Keywords
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Artic bacteria; Paenibacillus sp R4; crystal structure; esterase; psychrophilic
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Issue Date
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2018-06
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Citation
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Kim, Hyun, et al. 2018. "PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis". ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 74(6): 367-372.
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Abstract
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Esterases are very useful biocatalysts in industry: they hydrolyze esters and split them into a carboxylic acid and an alcohol. The psychrophilic esterase PsEst3 was obtained from Paenibacillus sp. R4, which was isolated from the active layer of the permafrost in Council, Alaska. PsEst3 was successfully overexpressed using a psychrophilic chaperonin co-expression system and was purified by nickel-affinity and size-exclusion chromatography. Recombinant PsEst3 was crystallized at 290 K using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 2.1 A ° resolution. The crystal was determined to belong to space group P4132 or P4332, with unit-cell parameters a = b = c = 145.33 A ° . Further crystallographic analysis needs to be conducted to investigate the structure and function of this esterase.
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URI
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https://repository.kopri.re.kr/handle/201206/10525
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DOI
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http://dx.doi.org/10.1107/S2053230X18007525
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Type
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Article
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Station
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Dasan Station
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Indexed
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SCIE
- Appears in Collections
- 2018-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (18-18) / Park, Hyun (PE18080)
2018-2018, Application study on the Arctic cold-active enzyme degrading organic carbon compounds (18-18) / Kim, Han-Woo (PN18082)
2017-2018, Application study on the Arctic cold-active enzyme degrading organic carbon compounds (17-18) / Lee, Jun Hyuck; Kim, Han-Woo (PN17083; PN18082)
2017-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (17-18) / Park, Hyun (PE17080; PE18080)
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