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PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis

Cited 2 time in wos
Cited 3 time in scopus
Title
PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis
Other Titles
북극 미생물 Paenibacillus sp R4 유래의 저온성 에스테라제 PsEst3: 결정화와 X-ray 결정 분석
Authors
Kim, Hyun
Park, Ae Kyung
Lee, Jun Hyuck
Shin, Seung Chul
Park, Hyun
Kim, Han-Woo
Subject
Biochemistry & Molecular BiologyBiophysicsCrystallography
Keywords
Artic bacteriaPaenibacillus sp R4crystal structureesterasepsychrophilic
Issue Date
2018-06
Citation
Kim, Hyun, et al. 2018. "PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis". ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 74(6): 367-372.
Abstract
Esterases are very useful biocatalysts in industry: they hydrolyze esters and split them into a carboxylic acid and an alcohol. The psychrophilic esterase PsEst3 was obtained from Paenibacillus sp. R4, which was isolated from the active layer of the permafrost in Council, Alaska. PsEst3 was successfully overexpressed using a psychrophilic chaperonin co-expression system and was purified by nickel-affinity and size-exclusion chromatography. Recombinant PsEst3 was crystallized at 290 K using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 2.1 A ° resolution. The crystal was determined to belong to space group P4132 or P4332, with unit-cell parameters a = b = c = 145.33 A ° . Further crystallographic analysis needs to be conducted to investigate the structure and function of this esterase.
URI
https://repository.kopri.re.kr/handle/201206/10525
DOI
http://dx.doi.org/10.1107/S2053230X18007525
Type
Article
Station
Dasan Station
Indexed
SCIE
Appears in Collections  
2018-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (18-18) / Park, Hyun (PE18080)
2018-2018, Application study on the Arctic cold-active enzyme degrading organic carbon compounds (18-18) / Kim, Han-Woo (PN18082)
2017-2018, Application study on the Arctic cold-active enzyme degrading organic carbon compounds (17-18) / Lee, Jun Hyuck; Kim, Han-Woo (PN17083; PN18082)
2017-2018, Polar Genomics 101 Project: Genome analysis of polar organisms and establishment of application platform (17-18) / Park, Hyun (PE17080; PE18080)
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